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Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1986
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Remarks:
The report is not sufficiently documented. Details on study performance are missing. Hence the report cannot be used for assessment.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
other: determination of bacterial toxicity with Pseudomonas putida according to Bringman and Kühn
Version / remarks:
not specified
Deviations:
not specified
Principles of method if other than guideline:
Method: other: Bringmann-Kühn, Inhibition of cell growth of Pseudomonas putida
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1-chlorododecane
EC Number:
203-981-5
EC Name:
1-chlorododecane
Cas Number:
112-52-7
Molecular formula:
C12H25Cl
IUPAC Name:
1-chlorododecane
Test material form:
liquid
Details on test material:
Test substance: as prescribed by 1.1 - 1.4

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
The test substance is directly added to the solution of bacteria.

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Solution with bacteria

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

pH:
7.0-7.5
Nominal and measured concentrations:
concentrations of 50 mL/L, 25 mL/L, 10 mL/L, and 5 mL/L
Details on test conditions:
Type: aquatic
A stock solution has been prepared and has been diluted 1:2, 1:4, and 1:8. Corresponding to 50, 25,10, and 5 mL/L test substance concentrations.
The test substance solution has been directly added to the bacteria. The absorbance of the bacteria solution has been determined after 48 h incubation.

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
33 g/L
Nominal / measured:
nominal
Basis for effect:
growth inhibition
Details on results:
The test substance started to inhibit the cell growth of Pseudomonas putida at a concentration of 38 mL/L, corresponding to 33 g/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified