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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report date:
1982

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPP 81-1 (Acute Oral Toxicity)
Deviations:
no
GLP compliance:
no
Remarks:
study performed prior to implementation of GLP
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Reaction mass of 1-chlorodecane and 1-chlorododecane
EC Number:
907-241-9
Molecular formula:
C10H21Cl and C12H25Cl
IUPAC Name:
Reaction mass of 1-chlorodecane and 1-chlorododecane
Test material form:
other: liquid

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Age at study initiation: 6-8 weeks
- Weight at study initiation: males 157-202 g, females 152-192 g
- Fasting period before study: overnight
- Housing: 5 animals/cage
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: 1 week

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22°C +/- 2°C
- Humidity (%): 55% +/- 10%
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: From: 31.08.1981 To: 17.12.1981

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
polyethylene glycol
Details on oral exposure:
Single oral intubation via gavage to animals fasted overnight.
A dilution of the test compound was prepared with the help of a homogenizer (Ultra-Turrax) and kept stable during treatment with a magnetic stirrer. In the doses above 5000 mg/kg bw the test item was applied as a concentrate.
Doses:
500, 3000, 5000, 10000 and 20000 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: at 1, 2, 3, 5 and 24 h after dosing and once daily thereafter
- Frequency of weighing: day 0 (prior to dosing), day 7 and day 14
- Necropsy of survivors performed: yes

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 20 000 mg/kg bw
Based on:
test mat.
Mortality:
During the study period no mortality was observed.
Clinical signs:
other: The main symptoms observed were: sedation, dyspnoea, exophthalmos, curved position, diarrhoea and ruffled fur.
Gross pathology:
No pathological organ changes were observed.

Applicant's summary and conclusion

Interpretation of results:
study cannot be used for classification
Remarks:
Migrated information
Conclusions:
The acute oral LD50 of the test item in rats of both sexes observed over a period of 14 days was estimated to be greater than 20.000 mg/kg bw.