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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented publication which meets basic scientific principles.

Data source

Reference
Reference Type:
publication
Title:
Acute toxicity to Daphnia pulex of six classes of chemical compounds potentially hazardous to Great Lakes aquatic biota
Author:
Smith, S. B.; Savino, J. F.; Blouin, M. A.
Year:
1988
Bibliographic source:
J. Great Lakes Res. 14 (4): 394-404

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
other:
Version / remarks:
American Society of Testing and Materials 1980. E729-80
Deviations:
not specified
Qualifier:
according to guideline
Guideline:
other:
Version / remarks:
US EPA 1975. Report No. 660/3-75-009
Deviations:
not specified
Principles of method if other than guideline:
the test has been conducted in accordance to the standards of American Society of Testing and Materials (1980) No. E729-80 and US EPA (1975) Report No. 660/3-75-009.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1-chlorododecane
EC Number:
203-981-5
EC Name:
1-chlorododecane
Cas Number:
112-52-7
Molecular formula:
C12H25Cl
IUPAC Name:
1-chlorododecane
Specific details on test material used for the study:
CAS No. 112-52-7
Mol Wt. 205

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
not specified
Details on test solutions:
Solvent control is mentioned (acetone), but not specified if used for substance
Reconstituted hard water.

Test organisms

Test organisms (species):
Daphnia pulex
Details on test organisms:
Daphnia pulex cultured in reconstituted hard water at 20°C fed with a mix of green algae species.
Less than 24 h old
no feeding during test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
total hardness 160-180 mg/L as CaCO3
Dissolved oxygen:
dissolved oxygen concentration 8-9 mg/L
Nominal and measured concentrations:
five concentrations, range not further specified
Details on test conditions:
TEST SYSTEM
- Test vessel: beakers in water bath at 20°C
- No. of organisms per vessel: min. 10 Neonates/ test vessel
- Control and solvent control

EFFECT PARAMETERS MEASURED: immobilisation after 48 h
VEHICLE CONTROL PERFORMED: not specified
Reference substance (positive control):
yes
Remarks:
p,p' DDT

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.013 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other:
Remarks:
+/- SE 0.0019 mg/L
Results with reference substance (positive control):
DDT showed an EC50 of 0.0011 mg/L after 48 h.
Reported statistics and error estimates:
EC50 value were calculated by probit analysis and analysis of variance (ANOVA).

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The EC50 of the test substance has been determined to be 0.013 mg/L and has been considered as very highly toxic for invertebrates in comparison to other alkyl halides.

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