Hexylamine

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Scientifically acceptable study report, comparable to guideline with acceptable restrictions (due to reduced reporting in times before GLP).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1969

Report date:

1969

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

only 7 days observation period

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Hexylamine

EC Number:

203-851-8

EC Name:

Hexylamine

Cas Number:

111-26-2

Molecular formula:

C6H15N

IUPAC Name:

hexan-1-amine

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Willi Gassner, Sulzfeld, Germany
- Age at study initiation: young adults
- Mean weight at study initiation (± SD): males: 168.4 ± 29 g, females: 139.8 ± 18 g

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: aqueous emulsion containing Traganth

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 2; 4; 8 and 20 % (v/v)
- Amount of vehicle: 8; 10; 12.5; 16 mL/kg bw

MAXIMUM DOSE VOLUME APPLIED: 16 mL/kg bw

Doses:

200; 250; 320; 400; 800; 1600 µL/kg bw (corresponding to 152.84, 191.05, 244.54, 305.68, 611.36, 1222.72 mg/kg bw; calculated using the density of 0.7642)

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: Body weight was determined before the beginning of the study for dose calculation.
- Other examinations performed: Observation of clinical signs was several times on the day of administration and once daily afterwards with the exception of weekends and on holidays.
- Necropsy of survivors performed: yes

Statistics:

On the basis of the observed lethality, the LD50 value was estimated or determined using a graphical evaluation of the dose response curve on probability paper.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

approximate LD50

Effect level:

ca. 244 mg/kg bw

Based on:

test mat.

Remarks on result:

other: corresponding to the original value of ca. 320 mm3, calculated with density of 0.7642

Mortality:

Results are given as dead animals/total number of animals at this dose at 1 h / 24 h / 48 h / 7 days after dosing:
Males
153 mg/kg bw: 0/10, 0/10, 0/10, 0/10
192 mg/kg bw: 0/10, 0/10, 0/10, 0/10
245 mg/kg bw: 0/10, 1/10, 0/10, 0/10
306 mg/kg bw: 0/10, 7/10, 7/10, 7/10
613 mg/kg bw: 10/10, 10/10, 10/10, 10/10
1226 mg/kg bw: 10/10, 10/10, 10/10, 10/10

Females
153 mg/kg bw: 0/10, 0/10, 0/10, 0/10
192 mg/kg bw: 0/10, 2/10, 2/10, 2/10
245 mg/kg bw: 1/10, 4/10, 4/10, 4/10
306 mg/kg bw: 0/10, 10/10, 10/10, 10/10
613 mg/kg bw: 10/10, 10/10, 10/10, 10/10
1226 mg/kg bw: 10/10, 10/10, 10/10, 10/10

Clinical signs:

other: 153 mg/kg bw: Approximately 5 min after gavage crouched position, apathy and shallow respiration were observed. On the following days no abnormalites were detected. 192 - 245 mg/kg bw: Immediately after gavage increased disposition to develop convulsions,

Gross pathology:

Sacrificed animals: no abnormality detected.

Other findings:

No other fndings

Any other information on results incl. tables

Table 1 Acute oral toxicity

 

Dose* [mg/kg bw]	Mortality
	N	%	Time of death
Males
152.84	0/10	0	-
191.05	0/10	0	-
244.54	1/10	10	Within 24 hours
305.68	7/10	70	Within 24 hours
611.36	10/10	100	Within 24 hours
1222.72	10/10	100	Within 1 hour
Females
152.84	0/10	0	-
191.05	2/10	20	Within 24 hours
244.54	4/10	40	Within 1 to 24 hours
305.68	10/10	100	Within 24 hours
611.36	10/10	100	Within 24 hours
1222.72	10/10	100	Within 1 hour

* original doses (200, 250, 320, 400, 800 and 1600 mm3) converted using the densitiy of 0.7642

N= number of dead animals / number of animals used