Diethylammonium chloride

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented report which meets basic scientific principles.

Data source

Reference

Reference Type:

publication

Title:

COMPARATIVE ACUTE TOXICITY AND PRIMARY IRRITANCY OF VARIOUS CLASSES OF AMINES

Author:

Roy C. Myers, Bryan Ballantyne

Year:

1997

Bibliographic source:

Toxic Substance Mechanisms 16(2): 151-194

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

diethylamine (CAS: 109-89-7)

IUPAC Name:

diethylamine (CAS: 109-89-7)

Test material form:

other:

Details on test material:

- Name of test material (as cited in study report): diethylamine

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

not specified

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure chamber volume: 9 litres
- System of generating vapour: The test material was pumped into the top of a vertical glass evaporator tube, which was heated sufficiently to cause vaporization. Dried air was introduced through the bottom of the tube (counter-current to the sample flow) and the resultant vapor-containing atmosphere passed to the exposure chamber.

TEST ATMOSPHERE
- Brief description of analytical method used: Chamber concnetrations were determined by adjustment of sample and/or air flow rates.
- Samples taken from breathing zone: no

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

4 h

Concentrations:

4000 and 8000 ppm (nominal), corresponding to 18000 mg/m3 and 36000 mg/m3 , respectively

No. of animals per sex per dose:

6 (f)

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: several times on the day of exposure and daily thereafter.
- Frequency of weighing: just before exposure and at sacrifice
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Key result

Sex:

female

Dose descriptor:

LC50

Effect level:

5 700 ppm

95% CL:

>= 4 600 - <= 7 000

Remarks on result:

other: equivalent to 17300 mg/m3 and 17.3 mg/L air

Mortality:

8000 ppm: 6/6 (3 animals within exposure, 1 animal after 2.5 h, 2 animals after 24 h)
4000 ppm: 0/6

Clinical signs:

other: 8000 ppm: gasping, nasal irritation, poor coordination, bloody nasal discharge, tonic convulsions. 4000 ppm: wet noses, eyes partly closed within 10 min, slight loss of coordination within 25 min.

Body weight:

normal development

Gross pathology:

lungs red, intestines yellow, gas and liquid filled.

Survivors: no effects

Other findings:

Inhalation, by rats, of a nominal concentration of 8000 ppm resulted in death within a few hours.

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

CLP: Acute Tox. 4, H332