Diethylammonium chloride

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to the attached analogue justification provided in IUCLID Section 13.

Reason / purpose for cross-reference:

read-across source

Parameter:

% degradation (test mat. analysis)

Remarks:

HPLC

Value:

100

Sampling time:

28 d

Remarks on result:

other: Source: 5.2.1-1

Parameter:

% degradation (TOC removal)

Value:

99

Sampling time:

28 d

Remarks on result:

other: Source: 5.2.1-1

Parameter:

other: BOD(NO2)

Value:

69

Sampling time:

28 d

Remarks on result:

other: Source: 5.2.1-1

Parameter:

other: BOD(NH3)

Value:

89

Sampling time:

28 d

Remarks on result:

other: Source: 5.2.1-1

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19 Sep - 17 Oct 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: Circular on Test Methods of New Chemical Substances (Japan), biodegradation test

Deviations:

no

GLP compliance:

yes

Remarks:

according to the GLP standards by the Japanese Ministry of Environment

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: cool and dark
- Stability under test conditions: verified at the beginning and at the end of the test by IR

Oxygen conditions:

aerobic

Inoculum or test system:

mixture of sewage, soil and natural water

Details on inoculum:

- Source of inoculum/activated sludge: In June 1988, sludge was sampled at the following 10 places in Japan: 1. Fukogawa city sewage plant, 2. Fukashiba industry sewage plant, 3. Nakahama city sewage plant, 4. Ochiai city sewage plant, 5. Kitakami river, 6. Shinano river, 7. Yoshino river, 8. Lake Biwa, 9. Hiroshima bay, and 10. Dookai bay. Sampling method: return sludge at sewage disposal plants was taken. Surface water and surface soil on the beach which is in contact with atmosphere were taken from rivers, lake and sea.
- Method of cultivation: 5 L of the filtrate of the supernatant of old activated sludge was mixed with 500 mL of the filtrate of the supernatant of new sludge and cultured at pH 7.0 ± 1.0 under sufficient aeration using filtered air. About 30 minutes after ceasing aeration to the sludge mixture, supernatant corresponding to about 1/3 of the whole volume was removed. Then the equal volume of dechlorinated water was added to the remaining portion and aerated again, followed by addition of synthetic sewage at a concentration of 0.1% (w/v). This procedure was repeated once every day. The culturing was carried out at 25 ± 2 °C. During the cultivation, appearance of the supernatant, precipitability, formation of flock, pH, dissolved oxygen concentration in the solution and temperature were checked and necessary adjustments were made. Microflora in the activated sludge was microscopically observed and sludge with no abnormal symptom was used for the test.
- Concentration of sludge: 30 mg/L

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Parameter followed for biodegradation estimation:

TOC removal

Parameter followed for biodegradation estimation:

test mat. analysis

Details on study design:

TEST CONDITIONS
- Composition of medium: 3 mL each of four stock solutions, as described in Japanese Industrial Standards K 0102-1986-21, are diluted to 1000 mL with purified water
- pH: 7.0
- pH adjusted: yes
- Suspended solids concentration: determined according to Method Japanese Industrial Standards (JIS) K 0102-1986-14.1

TEST SYSTEM
- Culturing apparatus: closed system oxygen consumption measuring apparatus (Coulometer: Ohkura Electric Co., Ltd.); 300 mL vessel, absorbent for evolving carbon dioxide Soda lime No .1 (extra pure reagent, Wako Pure Chemical Industries, Ltd.).
- Number of culture flasks/concentration: 3 (sludge + test sub.)
- Measuring equipment: Coulometer, Okhura Electric Co., Ltd.
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used: soda lime, extra pure, Wako Pure Chemical Industries, Ltd.

CONTROL AND BLANK SYSTEM
- Inoculum blank: 1 (sludge)
- Inoculum control: 1 (sludge + reference substance)
- Abiotic control: 1 (water + test substance)
- Toxicity control: no

Reference substance:

aniline

Parameter:

% degradation (test mat. analysis)

Remarks:

HPLC

Value:

100

Sampling time:

28 d

Parameter:

% degradation (TOC removal)

Value:

99

Sampling time:

28 d

Parameter:

other: BOD(NO2)

Value:

69

Sampling time:

28 d

Parameter:

other: BOD(NH3)

Value:

89

Sampling time:

28 d

Results with reference substance:

The oxygen consumption for aniline in the above used test system reached 72% and 78% degradation after 7 days and 14 days.

Table 1 Biodegradability

		Biodegradability
		Sample 2	Sample 3	Sample 4
BOD	No2	69	70	68
	NH3	89	91	88
TOC		99	99	100
HPLC		100	100	100

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test material is readily bidegradable under the conditions of this assay (OECD TG 301C).

Description of key information

readily biodegradable within 28 d (OECD 301C) based on read across data from CAS 109-89-7

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Additional information

No reliable data testing the ready biodegradability of Diethylammonium chloride (CAS 660-68-4) are available. Therefore, good quality data for the analogous substance, N-ethylethanamine (CAS 109-89-7), have been used as read across.

The available study (reference 5.2.1-1) testing the biodegradation of the analogue substance N-ethylethanamine (CAS 109-89-7) was conducted according to the OECD guideline 301 C under GLP conditions. The inoculum (mixture of sewage, soil and natural water) was exposed to 100 mg test item/L for 28 days. The substance was found to be readily biodegradable under tested conditions.

Based on the results of the analogue substance, Diethylammonium chloride (CAS 660-68-4) is considered readily biodegradable.