Disodium 1,1'-isopropylidenedi-p-phenylene bis[2-[[5-amino-3-methyl-1-(3-sulphonatophenyl)-1H-pyrazol-5-yl]azo]benzenesulphonate]

Endpoint summary

Description of key information

Short term toxicity tests are available for all three tropic levels (fish, invertebrates and aquatic plants). In a 48 h acute fish toxicity test, the test substance FAT 21015/Awas observed to have 48 h TL₅₀to Rainbow trout 12.5 mg/L. Similarly in a study conducted according to OECD Guideline 202, the 48 h EC₀& NOEC value of the test substance FAT 21015 toDaphnia magna was determined to be 25.4 mg/L. The EC₅₀and the EC₁₀₀value could not be calculated, but are clearly higher than 51.1 mg/L. Due to intrinsic properties of the substance, the test to determine the acute toxicity to aquatic algae and cyanobacteria cannot be performed. The dark coloration of the test medium and reduced light intensity in the medium modifying the conditions of test. Hence, to determine the toxicity to the aquatic plant Lemna minor, a semi-static growth inhibition test was performed. In a GLP-compliant OECD Guideline 221 study, Lemna gibba (Duckweed) exposed to the test item FAT 21015 recorded: 7 day ErC₅₀based upon the frond number is >100 mg/L. 7 day EyC50 based upon the frond numbers is >100 mg/L. 7 day ErC₅₀based upon the frond dry weight is >100 mg/L. 7 day EyC₅₀ based upon the frond dry weight is >100 mg/Lwhile NOEC for growth rate of frond number as well as dry weight was 12.5 and 100 mg/L respectivelyTherefore, it can be concluded that there were no toxic effects at saturation. A toxicity study of FAT 21015 to activated sludge was determined in a 3-hours respiration inhibition test.The IC₅₀(3h) value of FAT 21015 is greater than 320 mg/L.