Disodium 1,1'-isopropylidenedi-p-phenylene bis[2-[[5-amino-3-methyl-1-(3-sulphonatophenyl)-1H-pyrazol-5-yl]azo]benzenesulphonate]

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24 February 1994 to 02 March 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.5 (Degradation: Biochemical Oxygen Demand)

Deviations:

yes

Remarks:

Aerate over night after addition of the inoculum

GLP compliance:

yes (incl. QA statement)

Remarks:

Swiss GLP

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Batch No.of test material: 1
- Expiration date of the lot/batch: 10/98
- Purity: 80 %

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Solubility and stability of the test substance in the solvent/vehicle: Miscible:

FORM AS APPLIED IN THE TEST (if different from that of starting material) solid

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Seeding water of an urban waste water

Duration of test (contact time):

5 d

Reference substance:

other: Glucose/Glutamic acid solution

Preliminary study:

None

Test performance:

None

Remarks on result:

not measured/tested

Key result

Parameter:

BOD5

Value:

0 mg O2/g test mat.

Results with reference substance:

The BOD5 of Glucose/Glutamic acid solution is 193 mg O2/I

Validity criteria fulfilled:

yes

Interpretation of results:

other: BOD5 has been determined

Conclusions:

The BOD5 of FAT 21015/B is: 0 mg O2/g test mat.

Executive summary:

The biochemical oxygen demand of FAT 21015/B is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of microorganisms. The test substance was tested in concentrations from about 6 to 800 mg/l. The reference substance D(+)-Glucose / L-Glutamic acid was tested in a concentration of 20 ml/I. Based on the findings from the study, the BOD₅ of FAT 21015 is 0 mg O₂/g test mat while that of refernce substance (Glucose/Glutamic acid solution) is 193 mg O2/I.

Reference 2

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 February 1994 to 15 February 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Deviations:

yes

Remarks:

Mean of 2 determinations instead of 3

GLP compliance:

yes (incl. QA statement)

Remarks:

Swiss GLP

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Batch No.of test material: 1
- Expiration date of the lot/batch: 10/98
- Purity: 80%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Solubility and stability of the test substance in the solvent/vehicle: Miscible:

FORM AS APPLIED IN THE TEST (if different from that of starting material) solid

Oxygen conditions:

aerobic

Inoculum or test system:

natural water

Details on inoculum:

None

Duration of test (contact time):

2 h

Reference substance:

other: potassium hydrogen phthalate solution

Preliminary study:

None

Remarks on result:

not measured/tested

Details on results:

None

Key result

Parameter:

COD

Value:

1 289 mg O2/g test mat.

Results with reference substance:

The COD of Potassium hydrogen phthalate is 199 mg 02/l

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The COD of FAT 21015/B is 1289 mg O2/g.

Executive summary:

A predeterminated amount of FAT 21015/B dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 h. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. The test was carried out according to DEV DIN 38'409 - H 41-1 (1980) corresponding to 92/96/EEC C.6. The test substance was tested with a sample weight of 2.5 mg. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/I. Based on the findings, the COD of FAT 21015/B was determined as 1289 mg O₂/g.

Description of key information

A study was performed to determine the ready biodegradation of FAT 21015/B. The Chemical Oxygen Demand of FAT 21015/B was determined to be 1289 mg/g O2, whereas the Biological Oxygen Demand of FAT 21015/B was determined to be 0 mg/g O2. In the supporting studies, the Chemical Oxygen Demand of FAT 21015/A was determined to be 1120 mg/g O2, whereas the Biological Oxygen Demand of FAT 21015/A was determined to be 82 mg/g O2.

The relevant BOD5/COD quotient is <0.5 , therefore the substance is not indicative of rapid degradation. Taking these data into consideration, FAT 21015 is considered to be not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Type of water:

freshwater