Bicyclo[3.1.1]hept-2-ene, 2,6,6-trimethyl-, phosphosulfurized

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 30 November 1992 to 04 December 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

characterization and analysis of test material not documented

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
no data

Analytical monitoring:

no

Details on sampling:

no data

Vehicle:

no

Details on test solutions:

None

Test organisms (species):

Cyprinodon variegatus

Details on test organisms:

TEST ORGANISM
- Common name: Sheepshead Minnow
- Source: Aquatic Biosystems, Inc., Fort Collins, CO
- Age at study initiation (mean and range, SD): ~13-15 weeks
- Feeding during test: Yes minus 24 hours before study start
- Food type: Tetramin flake fish food and frozen adult brine shrimp (Artemia sp.)
- Amount: 120

ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: Tetramin flake fish food and frozen adult brine shrimp (Artemia sp.)
- Health during acclimation (any mortality observed): Significant mortality occurred during the 4 days after the fish were received. Believed to have been caused by ammonia levels spiking in the tank and biofilter

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

The Mean Weight of the control fish at termination was 0.341g (sd=0.098)
The Mean total length of the control fish at termination was 2.8cm (sd=0.2)

Hardness:

No data

Test temperature:

The temperature was in the range of 21.3-21.8°C (See attached background material)

pH:

The pH was in the range of 7.4-8.3 (See attached background material)

Dissolved oxygen:

The dissolved oxygen was in the range of 5.7-7.8mg/l (See attached background material)

Salinity:

The salinity was approximately 25 ±2%

Nominal and measured concentrations:

Nominal concentrations: control (0), 0.625, 1.250, 2.500, 5.000, 10.000 g/L each with 2 replicates

Details on test conditions:

TEST SYSTEM
- Test vessel: 19 litre glass tank
- Type: open
- Material, size, headspace, fill volume: Artificial seawater prepared by adding available salts to dialyzed water
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Prepared by adding available salts to dialyzed water
- Total organic carbon: See method tables
- Metals: None detected
- Pesticides: None detected
- Chlorine: <0.2 mg/l
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark
- Light intensity: 65.67 to 67.57 footcandles
- Due to a computer malfunction on the 1st December 1992 the water quality was not measured. However, the temperature was taken by hand and believed to be within the specified range.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Not calculable

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 10 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Not calculable

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 10 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Not calculable

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 10 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Not calculable

Details on results:

At the termination of the test it was noticed that the test material was floating on the surface of the test chambers and they were all cloudy in appearance. White particles were observed in the 2.5g/L replicate-2 and the 5.0g/L replicate-1 chambers whilst white globules were seen on the floor of the 10.0g/L replicate-1 tank.

Results with reference substance (positive control):

No positive control

Reported statistics and error estimates:

None

Sublethal observations / clinical signs:

See background material.

Validity criteria fulfilled:

yes

Conclusions:

No mortality was observed from the test substance exposure to Cyprinodon variegatus over 96 hours.

Executive summary:

The test substance was tested for aquatic toxicity according to OECD Guideline 203. The sheepshead minnow, Cyprinodon variegatus, was used as the test system due to its historic use in safety evaluation. Two replicates, each consisting of 10 fish were exposed to five different concentrations of the test substance for 96 hours. At the termination of the test there were no mortaly in any of the tanks indicating an LC50 of >10 g/L. However, it was noticed that the test material was floating on the surface of the test chambers and they were all cloudy in appearance. White particles were observed in the 2.5 g/L replicate-2 and the 5.0 g/L replicate-1 chambers whilst white globules were seen on the floor of the 10.0 g/L replicate-1 tank. This led to fewer fish being used for the end results due to the low bioavailability of the test compound caused by its low water solubility.

Description of key information

The 96 hour LC50 of the registered substance was discovered to be greater than 10 g/L for marine water fish species Cyprinodon variegatus. As 10 g/L was the highest concentration tested it is proposed to take the conservative approach and use this concentration for the purpose of the classification. However, even with using this approach, bicyclo[3.1.1]hept-2 -ene, 2,6,6 -trimethyl-, phosphosulfurized, is not considered toxic to fish based on the results of this study.

Key value for chemical safety assessment

Marine water fish

Marine water fish

Effect concentration:

10 g/L

Additional information

The acute toxicity of the registered substance to marine fish was determined according to OECD guideline 203 (Fish acute Toxicity test). The species used for the study was Cyprinodon variegatus (Sheepshead minnow) which was tested under static conditions for 96 hours. The test was carried out under controlled conditions with a temperature of 21.3 -21.8 °C with a light: dark cycle of 16:8 hours. Ten organisms were kept in each vessel with two vessels (replicates) used per concentration tested. The registered substance showed no toxicity to the tested species, with the LC50 being greater than the highest concentration tested (10 g/L). However, it was noticed that the test material was floating on the surface of the test chambers and they were all cloudy in appearance.