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EC number: 939-409-2 | CAS number: 1329-99-3
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.42 mg/L
- EC10 or NOEC for freshwater algae:
- 0.28 mg/L
Additional information
In the GLP Klimisch 1 study from Aniol (2013) the acute toxicity of Depanol I to algaeDesmodesmus subspicatus (SAG 86.81)was determined in a static test under GLP. The test was performed according to OECD 201 (2006).
The test item Depanol I is a liquid poorly soluble in water and with volatile components. To avoid adverse physical effects of undissolved or emulsified test material on the algae, the test was carried out with aqueous extracts (WAF = Water Accommodated Fraction) at various loading rates in Alga-medium. Taking into account the volatile character of components of the test item, preparation of the Water Accommodated Fractions (WAFs) for the Alga Test was performed in closed glass vessels. The Water Accommodated Fractions (WAFs) for the Alga Test with Depanol I were prepared by stirring various amounts of the test item in Alga-medium for 24 h with magnetic stirrers. The WAFs were prepared in closed 2 L decanters. The test was started with a control and nominal concentrations of 1, 2.5, 5, 10, 25 and 50 mg/L loading rate. The concentrations were analyzed by GC-MS analyses. The mean measured concentrations were 0 (control), 17.7, 138.9, 266.4, 606.1, 1364, 1695 µg/L (only the four major constituents contributing to 96% of Depanol I were considered). During the test the concentrations of the test item was reduced by biotic and abiotic factors. For each test concentration 3 replicates were set up. For the control 6 replicates were set up. The test was performed in 250 mL Erlenmeyer flasks filled with 200 mL test solution. The test was started with 2500 cells/mL. The study met all validity criteria of the OECD 201.
The corresponding 72 h ErL50 value is 7.4 mg/L, the ErC50 is 0.42 mg/L.
This study is considered to be acceptable for the risk assessment.
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