Ethoprophos

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

September 1965

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1965

Report date:

1965

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Ethoprophos

EC Number:

236-152-1

EC Name:

Ethoprophos

Cas Number:

13194-48-4

Molecular formula:

C8H19O2PS2

IUPAC Name:

ethyl bis(propylsulfanyl)phosphinate

Test material form:

liquid

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Remarks:

albino

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: not specified
- Age at study initiation: 2.4 - 2.5 kg
- Weight at study initiation: not specified
- Housing: individually, in elevated metal cages
- Diet (ad libitum): Furina Rabbit Pellets
- Water (ad libitum): yes
- Acclimation period: not specified

ENVIRONMENTAL CONDITIONS
not specified

Test system

Vehicle:

unchanged (no vehicle)

Controls:

yes, concurrent no treatment

Amount / concentration applied:

0.1 mL

Duration of treatment / exposure:

not applicable, no washing

Observation period (in vivo):

1 h

Number of animals or in vitro replicates:

3 animals per sex

Details on study design:

Prior to application, the left eye of each rabbit was examined with 2.0% sodium fluorescein solution to detect any corneal lesions not otherwise visible, and only those animals with eyes free of irritation and corneal damage were used in this study.

A single application of 0.1 mL of the undiluted test material was made into the conjunctival sac of the left eye of each animal. The treated eye was held closed for 30 seconds following application.

The animals were observed for signs of eye irritation and systemic toxicity immediately following instillation and for signs of systemic toxicity during the period preceding death. Necropsies were performed on the animals following death.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

24/48/72 h

Max. score:

4

Remarks on result:

not determinable

Remarks:

due to acute toxicity (mortality) within 15 min

Results 2

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

24/48/72 h

Max. score:

3

Remarks on result:

not determinable

Remarks:

due to acute toxicity (mortality) within 15 min

Results 3

Irritation parameter:

chemosis score

Basis:

mean

Time point:

24/48/72 h

Max. score:

4

Remarks on result:

not determinable

Remarks:

due to acute toxicity (mortality) within 15 min

Results 4

Irritation parameter:

iris score

Basis:

mean

Time point:

24/48/72 h

Max. score:

2

Remarks on result:

not determinable

Remarks:

due to acute toxicity (mortality) within 15 min

Irritant / corrosive response data:

Immediately following application of the test item, moderate erythema and vascularization of the sclera and nictitating membrane were noted in all of the treated eyes.

Other effects:

MORTALITY
Within an additional 15 minutes toxic signs were noted in each animal and all animals were dead prior to the one-hour observation.

CLINICAL SIGNS
The following toxic signs were noted: preening, excessive masticatory movements, salivation, blinking, miosis, incoordination of limbs, rapid labored respiration, tremors, clonic and tonic convulsions, and death.

GROSS PATHOLOGICAL EXAMINATION
Necropsies revealed congestion of the lungs, liver, and kidneys and gaseous distention of the gastrointestinal tract.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

A single application of 0.1 mL of the undiluted test substance to the eyes of albino rabbits produced, in all three animals, death within one hour following instillation. Due to the high toxicity of the test item to the rabbit, eye irritating properties were not further investigated.

Executive summary:

In an experimental study from 1965, similar to OECD 405, 0.1 mL of the undiluted test substance was instilled to the left eye of 3 male and female rabbits. The remaining untreated eye served as a control. The animals were observed for signs of eye irritation and systemic toxicity immediately following instillation. Immediately following application of the test item, moderate erythema and vascularization of the sclera and nictitating membrane were noted in all of the treated eyes.

Within an additional 15 minutes toxic signs were noted in each animal and all animals were dead prior to the one-hour observation. The following toxic signs were noted: preening, excessive masticatory movements, salivation, blinking, miosis, incoordination of limbs, rapid labored respiration, tremors, clonic and tonic convulsions, and death. Necropsy was performed and revealed congestion of the lungs, liver, and kidneys and gaseous distention of the gastrointestinal tract.

Under the test condition, the test items eye irritation potential could not be evaluated as all animals died due to the high toxicity of the test item in the rabbit. Therefore, the eye irritating property of the test item was not further investigated.