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Ecotoxicological information

Long-term toxicity to fish

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Administrative data

Endpoint:
fish early-life stage toxicity
Remarks:
Larval survival and growth
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Referenceopen allclose all

Reference Type:
other company data
Title:
Unnamed
Year:
2008
Report Date:
2008
Reference Type:
study report
Title:
Unnamed
Year:
2001

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: US EPA Method 1000.0 (Larval survival and growth test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
HPLC with UV detector

Test solutions

Vehicle:
yes
Details on test solutions:
Dilution water was aged blended water characterized as having total hardness and alkalinity ranges as CaCO3 of 150mg/l and 152 mg/l, respectively, and specific conductance of 332 μS/cm.

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
in-house culture

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
7 d
Post exposure observation period:
none

Test conditions

Hardness:
150 mg/L
Test temperature:
24.5-25.3°C
pH:
7.69-8.27 in fresh solutions; 7.61-8.23 in expired solutions
Dissolved oxygen:
7.4 - 8.45 mg/L in fresh solutions; 4.9 - 7.13 mg/L in expired solutions
Salinity:
152 mg/L alkalinity
Conductivity:
332 uS/cm
Nominal and measured concentrations:
0, 0.63, 1.3, 2.5, 5.0 and 10 mg/L (nominal)
0, 0.355, 0.606, 1.28, 2.62, 5.01 mg/L (measured)
Details on test conditions:
Treatment vessels were glass beakers containing 250 ml of test solution. Ten fish larvae were added to each of three replicate beakers per treatment and control.

Minnows were fed three times daily with newly hatched brine shrimp and rotifers at a rate such that there was a small amount of food left uneaten at the next feeding. Test solutions were renewed daily by replacing ≥ 90% of each test solution with fresh solutions after removing as much of the feces and uneaten food from the remaining ~10% solution as possible. The expired water was pooled by test concentration for analysis.

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
7 d
Dose descriptor:
EC50
Effect conc.:
1.9 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.8 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
3.5 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
7 d
Dose descriptor:
NOEC
Effect conc.:
1.28 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
2.8 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
2.62 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
7 d
Dose descriptor:
LOEC
Effect conc.:
2.62 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
2.62 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
24 h
Dose descriptor:
LOEC
Effect conc.:
5.01 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
7 d
Dose descriptor:
EC50
Effect conc.:
1.4 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
7 d
Dose descriptor:
NOEC
Effect conc.:
0.355 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
7 d
Dose descriptor:
LOEC
Effect conc.:
0.606 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
The mean measured concentrations of the test substance averaged 39 to 64% of the nominal concentrations. Recoveries from quality control fortifications ranged from 67 to 93% of the nominal concentrations. All reported results are based on mean measured concentrations. Undissolved test substance was observed in the primary stock solutions; however, all test solutions appeared clear and colorless throughout the test with no visible particulates, surface films or undissolved test substance.

Fish mortality during the 7-day exposure period ranged from 0% at 0.355 mg/l and 1.28 mg/l to 100% at 5.01 mg/l. Control survival was 97%. Growth of fish surviving the 7-day exposure ranged from 0.2 mg to 1.2 mg. The mean growth per fish in the control was 1.3 mg. Clinical signs through the exposure period included spinal curvature, erratic swimming, discoloration in fish exposed to 2.62 mg/l. All fish in the 10-mg/l group died within the first day of exposure.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
ATBC had EC50 = 1.4 mg/L based on 7-day growth statistics; NOEC = 0.355 mg/L.
Executive summary:

ATBC had EC50 = 1.4 mg/L based on 7-day growth statistics; NOEC = 0.355 mg/L.