N,N-dimethylpyridin-4-amine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1977

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: U.S. Federal Hazardous Substances Act, Section 1500.3. Fed Reg Vol 38, No. 187, p 27014, 1973.

Deviations:

yes

Remarks:

; the skin of half the animals was abraded. Few animalsper dose group were tested than in OECD guideline.

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

N,N-dimethylpyridin-4-amine

EC Number:

214-353-5

EC Name:

N,N-dimethylpyridin-4-amine

Cas Number:

1122-58-3

Molecular formula:

C7H10N2

IUPAC Name:

N,N-dimethylpyridin-4-amine

Test material form:

solid: crystalline

Details on test material:

The test substance was material prepared in a company lab and recrystallized twice from benzene and three times from benzene/hexane into a fine white crystalline material. It had a constant freezing point of 11.6 degrees C, and a purity of 100% by GLC. The boiling point was 162.5 degrees C, solubility in water of 7.6 g at 25 degrees C, and a flash point of 230 degrees F. This is comparable to current commerical product.

Test animals

Species:

rabbit

Strain:

other: albino

Sex:

male/female

Details on test animals or test system and environmental conditions:

Two male and two female albino rabbits weighing between 2.5 and 3.5 kg each were employed in this study.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

water

Details on dermal exposure:

Test material was dosed as supplied.

Duration of exposure:

24 hours

Doses:

0.062, 0.125, 0.25, 0.50 g/kg.

No. of animals per sex per dose:

2 male and 2 female per dose group

Control animals:

not specified

Details on study design:

All animals had their backs clipped free of hair 24 h prior to testing. One half of the clipped animals had their backs abraded prior to dosing. The treated areas of the backs of the animals were covered with large gauze patches and an impervious material was wrapped snugly around the trunk of each animal. The dressings were removed after 24 h and any excess material was removed and the approximate amount of material remaining was noted. The animals were observed for a 14 day period for signs of toxicity and for mortalities. Gross autopsies were performed on all animals which died during the 14-day observation period.

Statistics:

no data

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

90 mg/kg bw

Based on:

test mat.

95% CL:

>= 62 - <= 125

Remarks on result:

other: Reported as 0.09 g/kg.

Mortality:

All animals dosed with concentrations over 0.062 g/kg died within the first 5-8 h after dosing.

Clinical signs:

other: Animals which died displayed coma.

Gross pathology:

No remarkable findings upon necropsy

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category II

Remarks:

Migrated information The LD50 is greater than 50 but less than 200 mg/kg bw. Criteria used for interpretation of results: EU

Conclusions:

The dermal LD50 in rabbits is 90 mg/kg bw, warranting an Acute Dermal Toxicity classification of Category 2.

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