2-Butanol, 2-methyl-, lithium salt (1:1)

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 2007-11-26 to 2007-11-29

Reliability:

1 (reliable without restriction)

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Version / remarks:

92/69/EEC

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
NA

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

For the analysis of the test item concentration, samples from the Algal Mineral Salts Test Medium solutions of the test concentration and the controls were taken at the start and at the end of the test. All test medium samples were taken from the approximate centre of the test vessels with mixing of the test media.

Test solutions

Vehicle:

no

Details on test solutions:

according to OECD guideline 201 (adopted 23rd March 2006)

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

NA

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Post exposure observation period:

NA

Test conditions

Hardness:

reconstituted Water.

Test temperature:

22.0 - 23.7 °C

pH:

pH 7.97 to 10.81 at test start
pH 8.80 to 9.37 at test end

Dissolved oxygen:

NA

Salinity:

NA

Nominal and measured concentrations:

The nominal test item concentrations were 1.0; 3.16; 10.0; 31.6 and 100.0 mg/L.
The deviation of the measured concentrations from the nominal values was less than 20 % during the study.

Details on test conditions:

NA

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

IC50

Effect conc.:

23.97 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 95 % conf. limits: 21.80 – 26.14

Effect concentrations 2

Duration:

72 h

Dose descriptor:

IC50

Effect conc.:

36.51 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95 % conf. limits: 32.35 – 40.68

Effect concentrations 3

Duration:

72 h

Dose descriptor:

IC50

Effect conc.:

22.89 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: yield

Remarks on result:

other: 95 % conf. limits: 20.84 – 24.93

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

3.16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 5

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

3.16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 6

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

3.16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: yield

Effect concentrations 7

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 8

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 9

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: yield

Details on results:

NA

Results with reference substance (positive control):

see results including tables and figures.

Reported statistics and error estimates:

NA

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

IC50 (72h) for growth rate: 36.51 mg/L.

Executive summary:

LTA had a statistically significant inhibitory effect on the growth based on areas under the growth curves, average specific growth rate and on the yield of Pseudokirchneriella subcapitata after the exposure period of 72 hours in the concentration range of 10.0 – 100.0 mg test item/L (Bonferroni t-Test, a=0.05). The 72-hour IC50 for growth rate was determined at the concentration of 36.51 mg test item/L.