2-Butanol, 2-methyl-, lithium salt (1:1)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 2007-11-27 to 2007-11-29

Reliability:

1 (reliable without restriction)

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

92/69/EEC

Deviations:

no

Principles of method if other than guideline:

NA

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
NA

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

before and after 48h.

Test solutions

Vehicle:

no

Details on test solutions:

NA

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

NA

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

NA

Test conditions

Hardness:

c(CaCO3) = 167.0 mg/L.

Test temperature:

19.9 – 20.2 °C

pH:

pH 7.40 to 10.54

Dissolved oxygen:

at least 6.2 mg/L or higher

Salinity:

n.a.

Nominal and measured concentrations:

Test concentrations: 6.25;12.5;25.0;,50.0;100.0 mg/L (nominal)
The analytically determined mean test item concentrations in the analysed test media varied in the range from 90 % to 104 % of the nominal value at
the start of the study and from 85 % to 94 % at the end of the study .

Details on test conditions:

NA

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

NA

Results with reference substance (positive control):

NA

Reported statistics and error estimates:

NA

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

The experiment is valid because the immobilisation of Daphnia magna in the control did not exceed 10 % and no Daphnia were trapped at the water surface.

Conclusions:

EC50 (48 h) > 100 mg/L or 97.6 mg test item/L (considering analytical determination - see above).

Executive summary:

The 48h-NOEC (highest concentration tested without toxic effects after the exposure period of 48 hours), respectively the 48h-EC0 of Lithium-tert-amoxide (LTA) to Daphnia magna was determined to be 50.0 mg test item/L.
The 48h-EC50 of Lithium-tert-amoxide (LTA) to Daphnia magna was determined to be 97.6 mg test item/L (considering analytical determination - see above).