methyl (2E)-{2-[(2,5-dimethylphenoxy)methyl]phenyl}(methoxyimino)ethanoate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study conducted in accordance with GLP principles

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): MEOE
- Analytical purity: 96% (analytical report No.: 06L00055)
- Test-substance No.: 04/0450-2

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Strain: HanRcc:WIST(SPF)
- Source: RCC Ltd Laboratory Animal Services
- Age at study initiation: female animals approx. 8-12 weeks
- Fasting period before study: Feed was withdrawn from the animals at least 16 hours before administration, but water was available ad libitum.
- Weight at study initiation: females 183 g (mean)
- Housing: Single in stainless steel wire mesh cages, type DK-III
- Diet (ad libitum): Kliba-Labordiaet, Provimi Kliba SA, Kaiseraugst, Basel, Switzerland
- Water (ad libitum): Tap water
- Acclimation period: at least 5 days for administration


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 30-70
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: 0.5% CMC-solution (cleaned sodlum carboxymethylcellulose) in doubly distilled water

Doses:

5000 mg/kg bw

No. of animals per sex per dose:

3 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Individual body weights shortly before application (day 0), weekly thereafter and at the end of the study; Recording of signs and symptoms several times on the day of application, at least once each workday for the individual animals; A check for any dead or moribund animal was made twice each workday and once on Saturdays, Sundays and on public holidays; Necropsy with gross-pathology examination on the last day of the observation period after killing with CO2.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Mortality:

No mortality occurred.

Clinical signs:

Clinical observation revealed impaired general state, dyspnoea and piloerection and were observed from hour 3 through to hour 5 after administration.

Body weight:

The mean body weights of the administration groups increased throughout the study period.

Gross pathology:

No macroscopic pathologic abnormalities were noted in the animals (3 females) examined at termination of the study.

Any other information on results incl. tables

Under the conditions of this study the median lethal dose of MEOE after oral administration was found to be greater than 5000 mg/kg body weight in rats.