methyl (2E)-{2-[(2,5-dimethylphenoxy)methyl]phenyl}(methoxyimino)ethanoate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Valid without restriction

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): 505-Oximether
- Substance type: pure
- Physical state: solid powder
- Analytical purity: 84.7 % w/w
- Impurities (identity and concentrations): water 6.6% w/w
- Lot/batch No.: 08/074 B

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Inoculum (concentration and source): Activated sludge from a laboratory waste water treatment plant, fed with municipal sewage.
- Mass: 30 mg/l (dry substance).
- Concentration of test chemical: 33 mg/l
- pre-acclimation conditions: the activated sludge was sieved and pre-aerated for about 48 hours before the test starts.
- Temperature of incubation(°C): 22 ± 2

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

33 mg/L

Based on:

test mat.

Details on study design:

Sampling frequency: The test substance is poorly soluble in the test medium, therefore no DOC elimination was determined. Measurement and calculation : Appropriate controls and blank system used: yes [BC = blank control; RS = reference substance; IH = inhibition control; ] Analytical method used to measure biodegradation: CO2 evolution

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

< 10

Sampling time:

28 d

Details on results:

RS-Freetext:
The test substance is poorly soluble in water, therefore no DOC-elimination was determined.

Kinetic of test substance (in %):
= 1 after 1 day(s)
= 0 after 7 day(s)
= 1 after 14 day(s)
= 1 after 21 day(s)
= 0 after 28 day(s)
Kinetic of control substance (in %):
= 61 after 7 day(s)
= 86 after 28 day(s)
Degradation products: not measured

Applicant's summary and conclusion

Interpretation of results:

other: The test substance is poorly soluble in water, therefore no DOC-elimination was determined.