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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Valid without restriction

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report date:
2005

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:

Name of the test substance: 505-Oximether
Purity: 84.7 % w/w

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test concentration was prepared by diluting an acetonic stock solution. The stock
solution will be prepared by weighing 0.00507 g test substance and diluting it in 100 mL
acetone (SI-, stock solution No 1). A second dilution was done: 1 mL SLI was added to
100 mL acetone (SL-2). The required amount (10 ml) of the acetonic stock solution (SL-2) was
given into 100 mL volumetric flasks. For the dilution steps from SL3 to SL6 each time 25 ml
of the corresponding SL solution was added to 50 ml acetone. After evaporating of the
acetone the volumetric flasks were fiIIed with test medium. The test substance was stirred in
M4 medium for about one day at 20 ± 2 0C. The stock solution appears colourless-clear.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- Test organisms: Daphnia magna Straus
- Source: originally supplied from "Institut National de Recherche Chimique appliquee, France", bred from 1978 in the Laboratories of BASF. b)
- Age: 2 - 24 hours old (starting with the 3rd breed of parent animals)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Details on test conditions:
- A synthetic fresh water was used for culture and test purposes. For the composition of this M4 medium see ISO 10706.
- Total hardness: 2,20 - 3,20 mmol/l
- Alkalinity up to pH 4.3: 0,80 - 1,00 mmol/l
- Molar ratio Ca:Mg: about 4 : 1
- pH value: 7,5-8,5
- Conductivity: 550 - 650 pS/cm
- Test temperature: 18 - 22 °C
- Exposure vessels: test tubes (glass) with flat bottom (nominal volume 20 ml)
- Light conditions: Artificial light, type warm white
- day:night rhythm = 16:8 h
- Number of Replicates: 4
- Individuals /replicate: 5
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.007 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
0.015 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.008 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

RS-Freetext:
Nominal concentrations in mg/L: control, 0.0031, 0.0063, 0.0125, 0.025,  0.05
Measured concentrations in mg/L: The analytical recovery rate in all  analyzed test solutions was below 80%. The effective concentrations  mentionied in the report are based therefore on the median analytical  recovery rate between Oh and 48h.

EC0 (48h): 0.00688 mg/l 
EC50 (48h): 0.00795 mg/l 
EC100 (48h): 0.015 mg/l 

Mobile daphnids after x hours:

Nom. Conc. (mg/l) / 0h / 24h / 48h
Control (0 mg/l)  / 20 / 20 / 20 
0.0031 mg/l       / 20 / 20 / 20 
0.0063 mg/l       / 20 / 20 / 20 
0.0125 mg/l 	  / 20 / 20 / 19 
0.025 mg/l	  / 20 /  3 /  0 
0.05 mg/l 	  / 20 /  0 /  0

Applicant's summary and conclusion

Validity criteria fulfilled:
yes