Hexahydro-4,7-methano-1H-indenyl acrylate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study, acceptable for assessment.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Reference 2

Reference Type:

other company data

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Version / remarks:

(adopted 1981)

Deviations:

yes

Remarks:

Only 0.05 mL applied (instead of 0.1 mL), observation period: only 8 days (instead of 21 days)

Principles of method if other than guideline:

The study was conducted following a protocol based on BASF-internal standards.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Hexahydro-4,7-methano-1H-indenyl acrylate

EC Number:

235-697-2

EC Name:

Hexahydro-4,7-methano-1H-indenyl acrylate

Cas Number:

12542-30-2

Molecular formula:

C13 H16 O2

IUPAC Name:

2-Propenoic acid hexahydro-4,7-methano-1H-indenyl ester

Details on test material:

- Name of the test substance used in the study report: Dihydrodicyclopentadienylacrylat
- Analytical purity: 99%

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.99 kg (male) and 2.82 kg (female)


ENVIRONMENTAL CONDITIONS
no details given

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated eye of the same animal

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.05 mL

Duration of treatment / exposure:

once

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): not rinsed


SCORING SYSTEM: Draize Scoring System


TOOL USED TO ASSESS SCORE: none

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

other: 24 - 48 hours

Score:

1

Max. score:

3

Reversibility:

fully reversible within: 5 days

Remarks on result:

other: (animals 1 and 2 had identical scores)

Results 2

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 24 - 48 hours

Score:

0

Max. score:

4

Reversibility:

other: no effects

Results 3

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 24 - 48 hours

Score:

0

Max. score:

2

Reversibility:

other: no effects

Results 4

Irritation parameter:

chemosis score

Basis:

mean

Time point:

other: 24 - 48 hours

Score:

0

Max. score:

4

Reversibility:

other: no effects

Any other information on results incl. tables

Mean scores after 24 and 48 h*:

 

Scores	24 h		48 h		mean
	Animal 1	Animal 2	Animal 1	Animal 2	Animal 1	Animal 2
Conjunctivae	1	1	1	1	1	1
Cornea	0	0	0	0	0	0
Chemosis	0	0	0	0	0	0
Iritis	0	0	0	0	0	0
Discharge	0	0	0	0	0	0

*72 hrs-scores were missing in the raw data.

 

 

 

Findings at test termination:

 

Scores	8 d
	Animal 1	Animal 2
Conjunctivae	0	0
Cornea	0	0
Chemosis	0	0
Iritis	0	0
Discharge	0	0

 

 

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information