Hexahydro-4,7-methano-1H-indenyl acrylate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Reference 2

Reference Type:

other company data

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Principles of method if other than guideline:

The study was conducted following a protocol based on BASF-internal standards.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Hexahydro-4,7-methano-1H-indenyl acrylate

EC Number:

235-697-2

EC Name:

Hexahydro-4,7-methano-1H-indenyl acrylate

Cas Number:

12542-30-2

Molecular formula:

C13 H16 O2

IUPAC Name:

2-Propenoic acid hexahydro-4,7-methano-1H-indenyl ester

Details on test material:

- Name of the test substance used in the study report: Dihydrodicyclopentadienylacrylat
- Analytical purity: 99%

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gaukler / Henning
- Sex: female/male
- Age at study initiation: no data
- Mean body weight at study initiation: female 3.06 kg; male: 3.3 kg
- Diet (ad libitum): Ssniff K, standard diet for rabbits, INTERMAST GmbH, Soest
- Water: ad libitum


ENVIRONMENTAL CONDITIONS
no details given

Test system

Type of coverage:

occlusive

Preparation of test site:

other: clipped

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated skin areas of the same animals

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): approx. 1 mL

Duration of treatment / exposure:

1 min, 5 min, 15 min, and 20 h

Observation period:

8 days

Number of animals:

2

Details on study design:

TEST SITE
- Area of exposure: flank and back, 2.5 x 2.5 cm
- Type of wrap if used: the test patch which was wetted with the test substance was applied to the dorsal skin of the rabbits by means of a gummed linen patch that was fastened by an adhesive dressing.


REMOVAL OF TEST SUBSTANCE
- Washing: yes, with lutrol or lutrol:water (1:1) in case of 1, 5, and 15 min application; no washing in case of 20 h application
- Time after start of exposure: after 1, 5, and 15 min


SCORING SYSTEM: Draize Scoring system

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

animal #1

Remarks:

(20 h exposure)

Time point:

other: 24-48 hours

Score:

3.5

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 2

Irritation parameter:

erythema score

Basis:

animal #2

Remarks:

(20 h exposure)

Time point:

other: 24 - 48 hours

Score:

3.5

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 3

Irritation parameter:

edema score

Basis:

animal #1

Remarks:

(20 h exposure)

Time point:

other: 24 - 48 hours

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 4

Irritation parameter:

edema score

Basis:

animal #2

Remarks:

(20 h exposure)

Time point:

other: 24 - 48 hours

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 5

Irritation parameter:

erythema score

Basis:

animal #1

Remarks:

(1, 5, 15 min exposure)

Time point:

other: 24 - 48 hours

Score:

2.5

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 6

Irritation parameter:

erythema score

Basis:

animal #2

Remarks:

(1, 5, 15 min exposure)

Time point:

other: 24 - 48 hours

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 7

Irritation parameter:

edema score

Basis:

animal #1

Remarks:

(1, 5, 15 min exposure)

Time point:

other: 24 - 48 hours

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 8

Irritation parameter:

edema score

Basis:

animal #2

Remarks:

(1, 5, 15 min exposure)

Time point:

other: 24 - 48 hours

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Any other information on results incl. tables

Mean erythema score after 24 and 48 h:

 

Exposure time	Concentration [%]	24 h		48 h		mean
		Animal 1	Animal 2	Animal 1	Animal 2	Animal 1	Animal 2
1 min	100	3**	3**	2*	3**	2.5	3.0
5 min	100	3**	3**	2*	3**	2.5	3.0
15 min	100	3**	3**	2*	3**	2.5	3.0
20 h	100	4*	3**	3*	4**	3.5	3.5

* extending beyond the area of application

** extending far beyond the area of application

 

 

Mean edema score after 24 and 48 h:

 

Exposure time	Concentration [%]	24 h		48 h		mean
		Animal 1	Animal 2	Animal 1	Animal 2	Animal 1	Animal 2
1 min	100	3**	3**	3*	3**	3.0	3.0
5 min	100	3**	3**	3*	3**	3.0	3.0
15 min	100	3**	3**	3*	2**	3.0	2.5
20 h	100	3*	3**	3*	3**	3.0	3.0

* extending beyond the area of application

** extending far beyond the area of application

 

 

 

 

 

Findings at test termination:

 

Exposure time	Concentration [%]	Skin findings
		Animal 1	Animal 2
1 min	100	desquamation	desquamation
5 min	100	desquamation	desquamation
15 min	100	desquamation	desquamation
20 h	100	desquamation	desquamation*

* extending beyond the area of application

 

Applicant's summary and conclusion

Interpretation of results:

irritating

Remarks:

Migrated information