Methyl anthranilate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from authoritative database

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

Determination of short-term toxicity of test chemical on Daphnia magna

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

Common Name: Water Flea
Organism Age: < 24h

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

18.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% C.I = 15.4mg/L - 21.3 mg/L

Duration:

48 h

Dose descriptor:

NOELR

Effect conc.:

5.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

not specified

Conclusions:

The EC50 and NOEL value of test chemical on Daphnia magna was determined to be 18.2mg/L and 5.5mg/L, respectively after 48h of exposure of test chemical.Since the test chemical is readily biodegradable the test chemical is considered to be nontoxic to Fish and cannot be classified according to CLP regulation.

Executive summary:

An experimental study was conducted to determine the short term toxicity of test chemical to aquatic invertebrates. The test organism used in this study was Daphnia magna less than 24h old. The experiment was conducted in the static system using freshwater. After the exposure of test chemical for 48 h, the effect concentration EC50 and NOEL was determined to be 1 8.2 mg/L and 5.5mg/L, respectively. Since the test chemical is readily biodegradable the test chemical is considered to be nontoxic to Fish and cannot be classified according to CLP regulation.

Description of key information

An experimental study was conducted to determine the short term toxicity of test chemical to aquatic invertebrates. The test organism used in this study was Daphnia magna less than 24h old. The experiment was conducted in the static system using freshwater. After the exposure of test chemical for 48 h, the effect concentration EC50 and NOEL was determined to be 18.2 mg/L and 5.5 mg/L, respectively. Since the test chemical is readily biodegradable the test chemical is considered to be nontoxic to Fish and cannot be classified according to CLP regulation.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

18.5 mg/L

Additional information

Various experimental studies have been reviewed from different sources for target chemical and its structurally similar read across chemical to determine the short term toxicity of test chemical to aquatic invertebrates and their results are summarized below.

The first study was reviewed from authoritative database in this study an experimental study was conducted to determine the short term toxicity of test chemical to aquatic invertebrates. The test organism used in this study was Daphnia magna less than 24h old. The experiment was conducted in the static system using freshwater. After the exposure of test chemical for 48 h, the effect concentration EC50 and NOEL was determined to be 18.2 mg/L and 5.5mg/L, respectively. Since the test chemical is readily biodegradable the test chemical is considered to be nontoxic to aquatic invertebrates and cannot be classified according to CLP regulation.

Next study was reviewed from the study report in this an experiment was conducted to determine the short term toxicity of test chemical to aquatic invertebrates according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The test organism used in this study was Daphnia magna less than 24h old. The stock solution of test chemical was prepared by dissolving test chemical in acetone at concentration of 100 mg/l. Test solutions of required concentrations were prepared by mixing the stock solution of the test chemical in reconstituted water. The nominal concentration used in the study were 10, 20, 30, 45, 100 mg/l. The experiment was conducted in static system and test chemical was exposed to Daphnia magna. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test chemical, in Daphnia magna was determined to be 43.2 mg/L on the basis of mobility inhibition effects in a 48 hour study.

 

 

Last study was reviewed from secondary source in this study an experiment was conducted to determine the short term toxicity of test chemical to aquatic invertebrates. The test organism used in this study was Daphnia magna. The experiment was conducted in the static system using freshwater. After the exposure of test chemical for 24 h, the effect concentration EC50 was determined to be 50 mg/L. Since the test chemical is readily biodegradable the test chemical is considered to be nontoxic to aquatic invertebrates and cannot be classified according to CLP regulation.

By considering results of all the studies mentioned above the EC 50 value of test chemical was determined to be in range from 18.2 mg/L to 50 mg/L. Since the test chemical is readily biodegradable the test chemical is considered to be nontoxic to aquatic invertebrates and cannot be classified according to CLP regulation.