Registration Dossier
Registration Dossier
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EC number: 205-132-4 | CAS number: 134-20-3
Endpoint summary
Administrative data
Description of key information
Biodegradation in water:
Study was to determine biodegradation rate of test chemical in water. Study was carried out for 20 days under the condition of optimal bacterial growth. Test chemical was purchased from Fluka Chemical Company. The initial concentration of test chemical used in the study was 50 mg/L. Test chemical concentration was determined by High performance liquid chromatography (HPLC). The HPLC system consisted of a Rainin HPXL solvent delivery system, Dynamax A1-2 autosampler equipped with a 2 0 4 fixed sample loop and a Dynamax UV-M ultraviolet detector. Quantitative analysis of test chemical was done by using Hewlett Packard 3390A recording integrator. Brucker MSL-400 (400 MHz) NMR spectrometer, Finnegan GC/MS spectrometer equipped with a Data General Nova computer system, a Shimadzu IR-435 infrared spectrophotometer and a Gilford 2600 ultraviolet spectrophotometer were used for the spectral analysis. Approximately 50 mg liter of two solution of test chemical were prepared in dechlorinated, charcoal-filtered Vessels which were stored open to natural inoculation in a water bath maintained at 13-15°C for seven days prior to initiation of the test. One tank received 1 g of sodium azide, a metabolic poison after seven days resulting in a final concentration of 0.1 g liter. There is no further treatment given to control tank. 100 ml of samples were drawn, sealed (to prevent further inoculation) and stored at 23°C in sterilized, sealed amber vials. Vials were stored under dark or light: dark (12: 12 h) conditions. Aliquots from all treatments were analyzed for test chemical content at intervals of 0, 0.2, 1, 2, 5, 6, 7, 8, 9 and 20 days post-treatment. Under conditions of optimal bacterial growth (warmth and darkness), biodegradation of test chemical was determined to be 100% in 20 days. Based on the test chemical analysis, test chemical determined to be readily biodegradable in water.
Biodegradation in water and sediments:
Estimation Programs Interface (EPI Suite) prediction model was run to predict the half-life in water and sediment for the test chemical. If released in to the environment, 32.9 % of the test chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemical in water is estimated to be 15days (360 hrs). The half-life (15 days estimated by EPI suite) indicates that the chemical was not persistent in water and the exposure risk to aquatic animals is moderate to low, whereas, the half-life period of test chemical in sediment was estimated to be 135 days (3240 hrs). However, as the percentage release of test chemical into the sediment is less than 1% (i.e., reported as 0.105%), indicates that test chemical is not persistent in sediment.
Biodegradation in soil:
Test was carried out to determine degradation of test chemical in environment. Study was carried out for 64 days using a 1% suspension of Niagara silt loam soil. Test chemical observed to be completely degraded in 64 days.
Additional information
Biodegradation in water:
Following different studies includes experimental study for the test chemical and for read-across analogues which is extracted by using mechanistic approach and functionally and structurally similar to the test chemical to observe the biodegradation rate of test chemical in water.
The principle of the first study was to determine biodegradation rate of test chemical in water. Study was carried out for 20 days under the condition of optimal bacterial growth. Test chemical was purchased from Fluka Chemical Company. The initial concentration of test chemical used in the study was 50 mg/L. Test chemical concentration was determined by High performance liquid chromatography (HPLC). The HPLC system consisted of a Rainin HPXL solvent delivery system, Dynamax A1-2 autosampler equipped with a 2 0 4 fixed sample loop and a Dynamax UV-M ultraviolet detector. Quantitative analysis of test chemical was done by using Hewlett Packard 3390A recording integrator. Brucker MSL-400 (400 MHz) NMR spectrometer, Finnegan GC/MS spectrometer equipped with a Data General Nova computer system, a Shimadzu IR-435 infrared spectrophotometer and a Gilford 2600 ultraviolet spectrophotometer were used for the spectral analysis. Approximately 50 mg liter of two solution of test chemical were prepared in dechlorinated, charcoal-filtered Vessels which were stored open to natural inoculation in a water bath maintained at 13-15°C for seven days prior to initiation of the test. One tank received 1 g of sodium azide, a metabolic poison after seven days resulting in a final concentration of 0.1 g liter. There is no further treatment given to control tank. 100 ml of samples were drawn, sealed (to prevent further inoculation) and stored at 23°C in sterilized, sealed amber vials. Vials were stored under dark or light: dark (12: 12 h) conditions. Aliquots from all treatments were analyzed for test chemical content at intervals of 0, 0.2, 1, 2, 5, 6, 7, 8, 9 and 20 days post-treatment. Under conditions of optimal bacterial growth (warmth and darkness), biodegradation of test chemical was determined to be 100% in 20 days. Based on the test chemical analysis, test chemical determined to be readily biodegradable in water.
Supporting study was carried out to determine biodegradation of test chemical in water. Test was conducted in accordance with EPA OTS 796.3260 (Ready Biodegradability: Modified Sturm Test) for 29 days. Activated sewage sludge was used as test inoculums for the study. The initial concentration of test chemical used in the study was 28 mg/L. Based on the carbon dioxide evolution, the percent degradation of test chemical was determined to be 62% in 29 days. Thus on the basis of determined percent degradation value, test chemical is considered to be readily biodegradable in water.
The next biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test chemical. Activated sludge was used as test inoculums for the study. Concentration of inoculum i.e., sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test chemical was determined to be 85, 99 and 100% degradation by O2 consumption (BOD (NH3)), TOC removal andtest material analysis byHPLC parameter in 14 days. Thus, based on percentage degradation, test chemical is considered to be readily biodegradable in water.
On the basis of above determined values, test chemical is considered to be readily biodegradable in water.
Biodegradation in water and sediment:
Estimation Programs Interface (EPI Suite) prediction model was run to predict the half-life in water and sediment for the test chemical. If released in to the environment, 32.9 % of the test chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemical in water is estimated to be 15days (360 hrs). The half-life (15 days estimated by EPI suite) indicates that the chemical was not persistent in water and the exposure risk to aquatic animals is moderate to low, whereas, the half-life period of test chemical in sediment was estimated to be 135 days (3240 hrs). However, as the percentage release of test chemical into the sediment is less than 1% (i.e., reported as 0.105%), indicates that test chemical is not persistent in sediment.
Biodegradation in soil:
Following different studies includes experimental and estimated study for the test chemical to observe the biodegradation rate of test chemical in soil.
Test was carried out to determine degradation of test chemical in environment. Study was carried out for 64 days using a 1% suspension of Niagara silt loam soil. Test chemical observed to be completely degraded in 64 days.
The half-life period of test chemical in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (EPI suite). If released into the environment, 66.2% of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of test chemical in soil was estimated to be 30 days (720hrs). Based on this half-life value of test chemical, it can be concluded that the test chemical was not persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to low.
Based on this half-life value of test chemical, it can be concluded that the test chemical was not persistent in the soil environment.
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