Methyl anthranilate

Hydrolysis

Link to relevant study record(s)

Reference

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

To determine the rate of hydrolysis of test chemical.

GLP compliance:

no

Radiolabelling:

not specified

Analytical monitoring:

not specified

Details on sampling:

-Test chemical was purchased from Fluka Chemical Company


Preparation:
-3 ml of test chemical was added to 300 ml of deionized water which had been sterilized via autoclave.
-The mixture was stirred for 12 h at 24°C.
-No. of aliquots: 3 (50ml of each)

Buffers:

0.2 M phosphate buffer media at pH 5, 7 and 9

Details on test conditions:

-Three aliquots were injected into three sterile solutions (50 ml of each) of 0.2 M phosphate buffer having pH 5.0, 7.0 and 9.0, respectively.
-Solutions were stored at 24.5°C in the dark.
-Samples of each solution were taken for HPLC analysis.

Positive controls:

not specified

Negative controls:

not specified

Transformation products:

not specified

% Recovery:

> 96 - < 100

pH:

5

Temp.:

25 °C

Duration:

38 d

Remarks on result:

other: other details not available

% Recovery:

> 93 - < 97

pH:

7

Temp.:

25 °C

Duration:

38 d

Remarks on result:

other: other details not available

% Recovery:

> 91 - < 97

pH:

9

Temp.:

25 °C

Duration:

38 d

Remarks on result:

other: other details not available

Remarks on result:

not measured/tested

Details on results:

Study was carried out to determine percent rate of hydrolysis of test chemical. The Percent recovery of test chemical after 38 days at pH 5, 7 and 9 was determined to be 100-96%, 97-93% and 97-91%, respectively.

Test chemical was not subject to hydrolysis at 25°C.

Validity criteria fulfilled:

not specified

Conclusions:

Study was carried out to determine percent rate of hydrolysis of test chemical. The Percent recovery of test chemical after 38 days at pH 5, 7 and 9 was determined to be 100-96%, 97-93% and 97-91%, respectively.

Executive summary:

A 38 days study was conducted to determine the rate of hydrolysis of test chemical at the temperature of 25°C. Test solution was prepared by adding 3ml of test chemical to 300ml of deionized water which was stirred for 12h. Phosphate buffer media was used at pH 5, 7 and 9. Hence, after 38 days the percent recovery of test chemical at pH 5, 7 and 9 was determined to be 100-96%, 97-93% and 97-91%, respectively. Therefore, from the above value, it can be said that the test chemical was non-hydrolysable.

Description of key information

A 38 days study was conducted to determine the rate of hydrolysis of test chemical at the temperature of 25°C. Test solution was prepared by adding 3ml of test chemical to 300ml of deionized water which was stirred for 12h. Phosphate buffer media was used at pH 5, 7 and 9. Hence, after 38 days the percent recovery of test chemical at pH 5, 7 and 9 was determined to be 100-96%, 97-93% and 97-91%, respectively. Therefore, from the above value, it can be said that the test chemical was non-hydrolysable.

Key value for chemical safety assessment

Half-life for hydrolysis:

5.5 yr

Additional information

Following different studies includes experimental and estimated studies for the test chemical to observe the hydrolysis of test chemical in water.

 

A 38 days study was conducted to determine the rate of hydrolysis of test chemical at the temperature of 25°C. Test solution was prepared by adding 3ml of test chemical to 300ml of deionized water which was stirred for 12h. Phosphate buffer media was used at pH 5, 7 and 9. Hence, after 38 days the percent recovery of test chemical at pH 5, 7 and 9 was determined to be 100-96%, 97-93% and 97-91%, respectively. Therefore, from the above value, it can be said that the test chemical was non-hydrolysable.

 

In next study, the base catalyzed second order hydrolysis rate constant of test chemical was determined using a structure estimation method. The second order hydrolysis rate constant of test chemical was determined to be 0.042 L/mol-sec with a half-life value of 5.5 yrs and 200 days at pH 7.0 and 8.0, respectively. Based on the half-life values, it is concluded that the test chemical is not hydrolysable.

 

Based on the above value, the test chemical determined to be non-hydrolysable.