Registration Dossier
Registration Dossier
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EC number: 203-309-0 | CAS number: 105-56-6
Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, well documented and reliable for assessment
Data source
Reference
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The toxicity of ethyl cyanoacetate is studied upon single i.p. injection into mice
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- Reference substance 001
- Details on test material:
- - Name of test material (as cited in study report): Cyanoessigsäure
- Physical state: liquid
-
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 20-30 g (male) 20-28 g (female)
- Fasting period before study: 15-20 h
- Diet (e.g. ad libitum): Mrh-Haltungsdiät
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- CMC (carboxymethyl cellulose)
- Remarks:
- 0.5 % aqueous preparation
- Details on exposure:
- - The applied volume was constant at 10 ml/kg
- Doses:
- 50; 200; 700; 2000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of weighing and observations: multiple times on the day of application and daily therafter (except weekends). animals were weighed on day 2-4, day 7 and 13
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 700 - < 2 000 mg/kg bw
- Mortality:
- 700 mg/kg bw: 0/10 animals died
2000 mg/kg bw: 9/10 animals died - Clinical signs:
- 50 mg/kg bw: stretching
200 mg/kg bw: redness of skin, stretching
700 mg/kg bw: staggering, stretching
2000 mg/kg bw: dyspnoe, apathy, staggering, convulsions, anaemic paleness, dyspnoe - Body weight:
- mean weights:
50 mg/kg bw: body weights developed within the normal range
200 mg/kg bw: day 0: body weights developed within the normal range.
700 mg/kg bw: day 0: 28 g (male) and 26 g (female); day 13: 34.0 g (male) and 25.4 g (female) - Gross pathology:
- No treatment related abnormalities were observed.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
