Ethyl cyanoacetate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl cyanoacetate

EC Number:

203-309-0

EC Name:

Ethyl cyanoacetate

Cas Number:

105-56-6

Molecular formula:

C5H7NO2

IUPAC Name:

ethyl 2-cyanoacetate

Details on test material:

Batch no. Tank 434
ID no. 3633/81410

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna Straus, strain IRCHA
max. age 24 hours

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20 ± 1°C

pH:

6 - 7.9 pH

Dissolved oxygen:

4.8 - 8.1 mg/l

Salinity:

2.5 mmol/L Ca/Mg ions proportion 4:1

Nominal and measured concentrations:

100, 140, 200, 280, 400, 560 and 800 mg/l

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

613 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

471 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

After an 24 hour exposure with potassium dichromate in concentrations of 0.9 and 1.9 mg/l, the immobility was at 0.9 mg/l 30% and at 1.9 mg/l 100%.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of Ethylcyanoacetat at 24 h is 613 mg/l and 48h 471 mg/l.

Executive summary:

The study was perfomed according EU Method C.2 (Acute Toxicity for Daphnia).

The animals were treated and observed for 48 hours. No animal in the control group were immobilisized. The EC50 of Ethylcyanoacetat at 24 h is 613 mg/l and 48h 471 mg/l.