Ethyl cyanoacetate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study and GLP

Data source

Reference

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Directive 87/302/EWG Algal inhibition test

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl cyanoacetate

EC Number:

203-309-0

EC Name:

Ethyl cyanoacetate

Cas Number:

105-56-6

Molecular formula:

C5H7NO2

IUPAC Name:

ethyl 2-cyanoacetate

Constituent 2

Reference substance name:

[SPEC][/SPEC][SYN][/SYN]

IUPAC Name:

[SPEC][/SPEC][SYN][/SYN]

Constituent 3

Reference substance name:

[CS]000000157834[/CS]

IUPAC Name:

[CS]000000157834[/CS]

Details on test material:

Batch no. Tank 434
ID no. 3633/81410

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Name: Scenedesmus subspicatus
- Strain: 86.81 SAG

Study design

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

24 ± 2°C

pH:

at beginning of the test: 7.1 - 7.8
at the end of the test: 3.5 - 8.7

Nominal and measured concentrations:

cell concentration at test start: ca. 20000 cells/ml
substance nominal concentrations: 10, 17, 30, 50, 80 und 140 mg/l

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

72.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

17.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

294.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Remarks on result:

other: at concentration of 140 mg/l

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

141.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: at concentration of 140 mg/l

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

29.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 6

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

673

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: at concentration of 140 mg/l

Effect concentrations 7

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

17 mg/L

Details on results:

During the 72 h the test substance hydrolysed. At concentrations from 30 mg/l the ph-value decreased to acid. An influence on the test results cannot be excluded.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Ethyl cyanoacetate was tested according to Directive 87/302/EWG Algal inhibition test and reached after 72 hours an EbC50 of 72.4 mg/l related to cell number.

Executive summary:

The results from this algal growth study in which the unicellular green algae Scenedesmus subspicatus (new name: Desmodesmus subspicatus) were treated with the test substance Ethyl cyanoacetate at the nominal concentrations of 10, 17, 30, 50, 80 and 140 mg/l are given below.

Ethyl cyanoacetate was tested according to Directive 87/302/EWG Algal inhibition test and reached after 72 hours an E_bC₅₀of 72.4 mg/l related to cell number and an E_rC₅₀ of 140 mg/l related to growth rate.

The NOEC at 72 hours was 17 mg/l.

During the 72 h the test substance hydrolysed. At concentrations from 30 mg/l the ph-value decreased to acid. An influence on the test results cannot be excluded.