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EC number: 932-165-8
CAS number: -
conditions of the present study Vinasses did not induce growth rate
reduction at a nominal concentration of 100 mg/L (NOEC), corresponding
to an average exposure concentration of 56 mg/L. Vinasses
induced 23% inhibition of yield at a nominal concentration of 100 mg/L,
i.e. the NOEC was below a loading rate of 100 mg/L corresponding to an
average exposure concentration of 56 mg/L. The
72 h-EC50 for both growth rate reduction and yield inhibition
was above a nominal concentration of 100 mg/L. Based on non-specific
analysis the 72 h-EC50 was determined to be > 56 mg/L.
Calculation of growth rate (section-by-section)
Growth rate (µ)
Growth rate reduction (%)
The mean CV for section-by-section specific growth rate was:
Table 2: Measured Total Organic Carbon content
Time of sampling (hours)
Date of sampling
Date of TOC analysis
Measured organic carbon conc. (mg/L)
Calculated conc. Vinasses (mg/L)
samples were used to measure the control due to an analytical error in
the lowest calibration point
EC50(72h) > 100 mg/L (nominal), EC50 > 56 mg/L (measured) - for growthNOEC(72h) = 100 mg/L (nominal), NOEC = 56 mg/L (measured) - for growth
The toxicity of Vinasses, residue of
fermentation towards aquatic algae was investigated in a study conducted
according to the OECD Guideline 201 “Alga, Growth Inhibition Test”. The
freshwater algae Pseudokirchneriella subcapitata was used as test
organism and exposed to a nominal concentration of 100 mg/L for 72 h.
Based on the TOC content of the test material (i.e. 25.96%) the
concentration of Vinasses in the aquatic samples was calculated and
corrected for the control. No acute effects were observed based on
growth rate and yield (EC50(72h) > 56 mg/L, measured, EC50(72 h): > 100
mg/L, nominal). Furthermore, under the conditions of the present study
Vinasses did not induce growth rate reduction at a nominal concentration
of 100 mg/L (NOEC), corresponding to an average exposure concentration
of 56 mg/L. However, the test substance induced 23% inhibition of yield
at a loading rate of 100 mg/L, i.e. the NOEC was below a loading rate of
100 mg/L corresponding to an average exposure concentration of 56 mg/L
Similar to observations in the acute
fish test on this substance, after two days of exposure onwards the
limit concentration was observed to contain undissolved particles. There
is no test on the water solubility of this test substance available, but
for the similar UVCB substance, Vinasses, residue of fermentation
containing biomass of Corynebacterium glutamicum, it was shown
that a large fraction of components with relatively high water
solubility is existent (OECD 105). However, the test substance was not
completely soluble in water since in all samples undissolved test
substance particles were observed. Nevertheless, large fractions
exhibited a very high water solubility (38g/L at a loading rate of
100g/L, pH 3.5-3.6). Therefore, it can be concluded, that the observed
hazyness and partly precipitations are rather a result of the variable
composition of this test substance, than of a limited water solubility.
It is likely that both bacterial activities together with the variable
composition of the substance resulted in the observed precipitation.
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