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EC number: 810-298-1
CAS number: 194934-66-2
Verification of Test Concentrations
Analysis of the fresh test preparations at 0 and 72 hours showed
measured test concentrations of 0.39 and 0.17 mg/L
respectively. Analysis of the old test media at 24 and 96 hours showed
measured test concentrations of 0.18 and 0.17 mg/L respectively. Given
these results, it was considered appropriate to calculate the results
based on the geometric mean measured test concentration only in order to
give a “worst case” analysis of the data.
The geometric mean measured test concentration was determined to
Nominal Test Concentration(% v/v Saturated Solution)
Geometric Mean Measured Test Concentration (mg/L)
0 – 24 Hour
72 – 96 Hour
There were no mortalities in 7 fish exposed to a geometric mean
measured test concentration of 0.22 mg/L for a period of 96
hours. Inspection of the mortality data gave the following results:
test was considered to be valid given that none of the control fish died
or showed signs of stress during the test and that the oxygen
concentration at the end of the test was greater than or equal to 60% of
ASV (6.1 mg O2/L) in the control and test vessels.
Observations on Test Item Solubility
test item preparations were observed to be clear colorless solutions
throughout the test.
Cumulative Mortality Data in the DefinitiveTest
NominalConcentration(% v/v Saturated Solution)
Geometric Mean Measured TestConcentration(mg/L)
Cumulative Mortality (Initial Population = 7)
Water Quality Measurements
Nominal Concentration(% v/v Saturated Solution)
0 Hours (Fresh Media)
24 Hours (Old Media)
24 Hours (Fresh Media)
48 Hours (Old Media)
48 Hours (Fresh Media)
72 Hours (Old Media)
72 Hours (Fresh Media)
96 Hours (Old Media)
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