Nickel dihydroxide

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable publication which meets basic scientific principles

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1982

Materials and methods

Principles of method if other than guideline:

see details in remarks on material and methods.

GLP compliance:

not specified

Type of assay:

micronucleus assay

Test material

Test material information

Constituent 1

Reference substance name:

nickel nitrate hexahydrate

IUPAC Name:

nickel nitrate hexahydrate

Details on test material:

- Name of test material (as cited in study report): nickel nitrate hexahydrate

Test animals

Species:

mouse

Strain:

Balb/c

Sex:

male

Details on test animals or test system and environmental conditions:

ENVIRONMENTAL CONDITIONS
- Temperature (°C):
- Humidity (%):
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light):


IN-LIFE DATES: From: To:

Administration / exposure

Route of administration:

intraperitoneal

Duration of treatment / exposure:

5 weeks

Frequency of treatment:

single application

Post exposure period:

5 weeks

Doses / concentrations

Remarks:

Doses / Concentrations:
28; 56; 112; 224 mg/kg bw
Basis:
nominal conc.

No. of animals per sex per dose:

5 (males only)

Control animals:

other: physiological salt solution

Positive control(s):

200 mg/kg bw cyclophosphamide

Results and discussion

Test results

Sex:

male

Genotoxicity:

negative

Toxicity:

yes

Remarks:

from 56 mg/kg bw onwards

Vehicle controls validity:

not specified

Negative controls validity:

valid

Positive controls validity:

valid

Any other information on results incl. tables

Doses of 112 and 224 mg/kg bw Ni(N03)2*6H20 were highly toxic, all animals dying immediately or a few hours after treatment. Two mice injected with 56 mg Ni(N03)2*6H20 died after 1 week and these doses, corresponding roughly to 12 mg/kg bw nickel, were used for subsequent experiments.

The yield of micronuclei (per 1000) in the nickel nitrate group was 3.20 + 0.58 compared to 2.60 + 0.24 in the control group. The positive control group (200 mg/kg cyclophospamide) showed a yield of 46.60 + 6.08. No figures are given for the NCE/PCE ratio.Ni(N03)2*6H20 did not increase the number of polychromatic erythrocytes with micronuclei above the control level whereas many polychromatic erythrocytes carrying micronuclei were observed in the animals treated with the positive control cyclophosphamide.