Nickel dihydroxide

Long-term toxicity to fish

Administrative data

Endpoint:

fish, juvenile growth test

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Peer reviewed data, acceptable for assessment.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

No information

Author:

Deleeebeeck et al.

Year:

2005

Reference 2

Reference Type:

secondary source

Title:

SIDS Initial Assessment Profile on Nickel and Nickel Compounds-Draft SIAR

Author:

OECD

Year:

2007

Bibliographic source:

SIDS Dossier -Draft

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 215 (Fish, Juvenile Growth Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Nickel dichloride

EC Number:

231-743-0

EC Name:

Nickel dichloride

Cas Number:

7718-54-9

IUPAC Name:

nickel(2+) dichloride

Details on test material:

- Name of test material (as cited in study report): NiCl2
- Analytical purity: reagent grade

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: Houghton Springs Fish Farm (United Kingdom)
- Age at study initiation: ca. 35 days post hatch
- Weight at study initiation: ± 200 mg

POST-HATCH FEEDING
- Frequency of feeding: During the first two weeks of the exposure to nickel, fish were fed daily at 4% of the
mean wet body weight measured at the start of the test.

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

26 d

Test conditions

Hardness:

ca. 180 mg CaCO3/L

Test temperature:

14.5 ± 1 °C

pH:

ca. 7.8

Dissolved oxygen:

> 90% of saturation

Details on test conditions:

TEST SYSTEM
- Test vessel: polyethylene aquarium
- fill volume: 15 L
- Type of flow-through (e.g. peristaltic or proportional diluter): not stated
- Renewal rate of test solution (frequency/flow rate): not stated
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): not stated
- No. of vessels per control (replicates): not stated



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
All test media were prepared using carbon filtered, deionized
water (conductivity < 2 μS/cm, DOC assumed to be zero) and chemicals (reagent grade)
purchased from VWR (Leuven, Belgium). The composition of the basic medium (test medium
with low concentrations of all major cations: 0.12 mM Ca, 0.12 mM Mg, 0.078 mM K, 0.595
mM Na) was based on the medium recommended for algal growth inhibition tests as described by
OECD guideline 201 (1996).


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: light cycle of 12L:12D


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The endpoints tested were growth reduction, mortality and behaviour.
For determination of dissolved nickel concentrations, samples were taken twice a week

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

LC10

Effect conc.:

0.164 - 1.548 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

element

Remarks:

Ni

Basis for effect:

mortality

Effect concentrations 2

Duration:

21 d

Dose descriptor:

LC10

Effect conc.:

0.25 - 2.43 mg/L

Conc. based on:

other: Nickeldihydroxid

Basis for effect:

mortality

Remarks on result:

other: result calculated for Nickeldihydroxid