Nickel dihydroxide

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Peer reviewed data, acceptable for assessment.

Data source

Reference

Reference 1

Reference Type:

secondary source

Title:

SIDS Initial Assessment Profile on Nickel and Nickel Compounds-Draft SIAR

Author:

OECD

Year:

2007

Bibliographic source:

SIDS Dossier -Draft

Reference 2

Reference Type:

publication

Title:

no informations Cited in SIDS (peer reviewed)

Author:

Deleebeeck et al 2005 & De Schamphelaere et al 2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Nickel dichloride

EC Number:

231-743-0

EC Name:

Nickel dichloride

Cas Number:

7718-54-9

IUPAC Name:

nickel(2+) dichloride

Constituent 2

Reference substance name:

Nickel chloride

EC Number:

253-399-0

EC Name:

Nickel chloride

Cas Number:

37211-05-5

IUPAC Name:

nickel(2+) dichloride

Details on test material:

- Name of test material (as cited in study report): NiCl2
- Analytical purity: reagent grade.

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: For each bioassay, the prepared test medium was then used as the dilution water to make a nickel concentration
series (added as NiCl2, 5 to 6 nickel concentrations and a control). Nickel concentrations in control
media were always below the method detection level (MDL) of the GF-AAS (see further), which is 3
μg/l.

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

25 °C

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmayer Flasks
- Material, size, headspace, fill volume: 100 ml size, each flask containing 50 ml test medium.
- Initial cells density: inoculated with 1.10E4 cells/ml of P. subcapitata.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
All test media were prepared by adding different volumes of stock solutions of CaCl2 and MgCl2 to ISO medium (ISO,
1989) in which Na2-EDTA (100 μg/l) was replaced by 32 μg/l artificial dissolved organic carbon
(Aldrich Humic Acid AHA, Sigma-Aldrich Chemie, Steinheim, Germany).


OTHER TEST CONDITIONS
- Adjustment of pH: yes, The pH in the tests was controlled by adding 0.75 g/l MOPS (3-N-morpholinopropanesulfonic acid) to the test solution.
- Light intensity and quality: light table, no more information.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.025 - 0.425 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

element

Remarks:

Nickel

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.039 - 0.67 mg/L

Conc. based on:

other: Nickeldihydroxid

Basis for effect:

growth rate

Remarks on result:

other: result calculated for Nickeldihydroxid