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EC number: 202-443-7
CAS number: 95-71-6
The test item, Methylhydroquinone, was
assayed for the mutagenicity by the Bacterial Reverse Mutation Test. The
test was performed according to EU method B.13/14 Mutagenicity – Reverse
mutation test using bacteria, which is analogous to the OECD Test
Guideline No. 471.
Four indicator Salmonella
typhimurium strains TA 98, TA 100, TA 1535, TA 1537 and one
indicator Escherichia coli WP2 uvrA strain were used. The test
item was dissolved in water for injection and assayed in concentratins
given further, which were applied to plates in volume of 0.1 mL.
mutagenicity experiments were performed as plate incorporation test
without and with metabolic activation using a
supernatant of rat liver (S9;
30 μL or 100 μL per plate)
and a mixture of cofactors by the
plate incorporation test with a dose range of 0.1, 0.3, 1.0, 3.0 and 10 mg
of first series of mutagenicity experiments were negative, but the test
item was cytotoxic in the highest concentration in Salmonella
typhimurium TA 98 only, so concentration in the second mutagenicity
must have been increased to subtoxic concentrations in the other
As the test
item was differentially cytotoxic for differrent indicator strains,
concentrations in the other mutagenicity experiments were differrent for Salmonella
typhimurium TA 98 (0.1-30 mg
per plate ) Salmonella typhimurium TA 100 and E. coli (concentrations
per plate) and Salmonella typhimurium TA 1535 and TA 1537
(concentrations 10-300 mg
per and 2.5-50 mg
Volume of S9 in
the subsequent experiments was increased from 30 to 50 μL
concurrent positive controls verified the sensitivity of the assay and
the metabolising activity of the liver preparations. Average revertant
colony counts for the vehicle controls were within the current
historical control range for the laboratory.
In the arrangement given above, the
test item, Methylhydroquinone, was non-mutagenic for all the used
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