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EC number: 202-443-7
CAS number: 95-71-6
The preliminary test of study CSL-18-0142.06 at room temperature
showed, that the water solubility of the test item was between 29.0 and
Table: Preliminary test for the water
Amount of test item [mg]
Total volume of added water [mL]
Time in ultrasonic bath [min]
The solubility of the test item in water at room temperature was
estimated to be between 29.0 and 65.3 g/L. Due to this result, the flask
method was performed.
For the main test three flasks were prepared with an amount of
test item and water, and one flask only with water. After stirring and
centrifugation the aqueous phases were diluted with double distilled
water and measured by means of HPLC. The solution of each flask has been
measured twice. The values represented in the table are rounded values.
The calculation was carried out with the original not rounded values.
Results of the flask method
measured value [mg/L]
154.5 / 155.6
153.4 / 152.8
144.6 / 144.4
0 / 0
aliquot of the flask [mL]
total volume after dilution [mL]
water solubility [g/L]
77.2 / 77.8
76.7 / 76.4
72.3 / 72.2
average water solubility [g/L]
mean value of the water solubility [g/L]
standard deviation [g/L]
relative standard deviation [%]
To verify the accuracy of the analytical method freshly prepared
standard solutions (prepared with the test item) were measured in
between the samples of the flask method.
Measurements of standard solutions
(nominal value [mg/L])
Measured value [mg/L]
The recovery rate of the measured standard solutions was in the
range from 94.5 % to 101.7 %, which shows a sufficient accuracy of the
According to OECD Test Guideline 105 (1995) and Regulation EC
No. 440/2008 Method A.6. the water solubility at 20 °C of the test item
Methylhydroquinone was determined to be:
cS = 75.4 g/L, RSD = 3.7 %.
The water-solubility of methylhydroquinone using the flask-method was
determined to be 75.4 g/L at 20°C.
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