Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 946-788-8
CAS number: -
(Lemna minor, 7d, frond
number) 141.40 mg/L (geom. mean of measured conc.)
This study was performed in order to
evaluate the toxicity of tehe test item towards Lemna minor following
the OECD Guideline 221 (2006).The study was performed using 5
concentrations ranging from 1.0 to 100 mg/L (nominal concentration).
Incubation time was 7 days. The frond number of each replicate was
determined at the beginning, at day 2 and 5 during the test and at the
end of the experiment. Additionally, the dry mass of 12 representative
fronds was determined at the beginning of the experiment. At the end of
the experiment the dry mass of each replicate was determined.
Growth rate µ and the yield were
determined from the frond number and the drymass at the respective
At the start and at the end of the
test, the content of the test item in the test solutions was determined
using a Photometer.
The measured concentrations lay
between 27 % and 49 % of the nominal concentrations at the beginning of
the test and between 23 % and 61 % of the nominal concentrations at the
end of the test. At the end of the test the measured concentration of
treatment 1 mg/L was lower than the LOQ. Therefore, the determination of
the results was based on the geometric mean of the measured. Half the
LOQ (0.05 mg/L) of the 7d value was used to calculate the geometric mean
of the 1 mg/L treatment.
The measured concentrations were
much lower than the nominal concentration. It is very likely that the
solubility in STEINBERG medium is lower than the solubility in
demineralized water. Due to a strong colour of the stock solution at the
concentration 100 mg/L, presence of undissolved test item could not be
observed. This can be stated as uncritical for the outcome of the study
because in the lower concentrations no undissolved test item was present
and also toxicity was observed and the biological results were based on
the real measured concentration.
The 7d-EC50s of
3,5-Dichlorophenol (1,3-Dichloro-5-hydroxybenzene, C6H4Cl2O,
CAS-No. 591-35-5) were determined in a separate reference test. The EC50value
of the growth rate lay within the desired range of 1.7 - 5.7 mg/L,
mentioned in the paper “OECD Lemna Growth Inhibition Test, Development
and ring testing of draft OECD test guideline”, Research and Development
Technical Report EMA 003, page 34.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again