Reaction products of diazotised Sodium Picramate coupled with Resorcinol, subsequently coupled with diazotised 4'-Amino-4-Nitrodiphenylamin-2-Sulfonic Acid, at the end metallized with Iron (II) sulphate heptahydrate, sodium and ammonium salts

Short-term toxicity to aquatic invertebrates

Description of key information

EC50 (48 h, Daphnia magna) 48 mg/L (geom. mean of measured conc.)

Additional information

A static test was performed according to the OECD Guideline 202 (2004) and the EU method C.2 of the Regulation EC 440/2008. 5 concentrations were used ranging from 4.6 to 100 mg/L (nominal concentration). For each test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours. After 24 and 48 hours, the immobilised Daphnia were counted.

Potassium dichromate K₂Cr₂O₇ was used as positive controlin a current reference study to assure that the test conditions are reliable.

At the beginning and at the end of the test, the content of the test item in the test solutions was determined using photometer-determination. The concentrations determined at the start of the test were between 45 and 81 % of the nominal concentration. At the end of the test the determined concentrations were between 94 and 108 % of the nominal concentration. Therefore, the determination of the biological results was based on geometric mean of measured concentrations.

All validity criteria were met.