Cyclopentane, methoxy-

Toxicity to terrestrial plants

Link to relevant study record(s)

Reference

Endpoint:

toxicity to terrestrial plants: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 2013-01-28 to 2013-06-18

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: SEPA, The Guidelines for the Testing of Chemicals, 299 Seed Germination/Root Elongation Toxicity Test.

Version / remarks:

2004

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Batch No.: 2820249
Purity: ≥ 99.9%

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 1.56%, 3.12%, 6.25%, 12.5%, 25%, 50% and 100% of the saturated solution
- Sampling method: Each concentration solution was piptted one sample.
- Sample storage conditions before analysis: analyzed directly without storage

Vehicle:

yes

Remarks:

Ultra pure water

Details on preparation and application of test substrate:

-Preparation:
70 g test sample were added to 2 L ultra- pure water and vigorously stirred (700 rpm) for 72 h followed by filtration with 0.45 µm membrane to prepare the water saturated solutions as the stock solution (measured to be 14500 mg/L) used in the test. The other test solutions were prepared by diluting the stock solution.
- Procedure:
Clean quartz sand (about 220 g) were added to homemade inert plastic boxes, then 60-65 mL test solution was poured to mixed to prepare the test matrix. The test plant seeds exposed in the test substrate surface. stamped and put into artificial climate chamber.

Species:

Glycine max (G. soja)

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Soybean
- Source of seed: obtained from Su Yang of Jung Su provience on March 17th, 2013
- Seed storage: in seeds keeper under 4°C and 10% humidity

Species:

Lycopersicon esculentum

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Tomato
- Source of seed: obtained from Su Yang of Jung Su provience on March 17th, 2013
- Seed storage: in seeds keeper under 4°C and 10% humidity

Species:

Cucumis sativus

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Cucumber
- Source of seed: obtained from Su Yang of Jung Su provience on March 17th, 2013
- Seed storage: in seeds keeper under 4°C and 10% humidity

Species:

Brassica oleracea var. capitata

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Cabbage
- Source of seed: obtained from Su Yang of Jung Su provience on March 17th, 2013
- Seed storage: in seeds keeper under 4°C and 10% humidity

Species:

other: Capsicum frutscans

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Peper
- Source of seed: obtained from Su Yang of Jung Su provience on March 17th, 2013
- Seed storage: in seeds keeper under 4°C and 10% humidity

Species:

Zea mays

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: Corn
- Source of seed: obtained from Su Yang of Jung Su provience on March 17th, 2013
- Seed storage: in seeds keeper under 4°C and 10% humidity

Species:

Daucus carota

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Carrot
- Source of seed: obtained from Su Yang of Jung Su provience on March 17th, 2013
- Seed storage: in seeds keeper under 4°C and 10% humidity

Species:

Triticum aestivum

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: Wheat
- Source of seed: obtained from Su Yang of Jung Su provience on March 17th, 2013
- Seed storage: in seeds keeper under 4°C and 10% humidity

Species:

Oryza sativa

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: Rice
- Source of seed: obtained from Su Yang of Jung Su provience on March 17th, 2013
- Seed storage: in seeds keeper under 4°C and 10% humidity

Species:

Lactuca sativa

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Lettuce
- Source of seed: obtained from Su Yang of Jung Su provience on March 17th, 2013
- Seed storage: in seeds keeper under 4°C and 10% humidity

Test type:

seed germination/root elongation toxicity test

Study type:

laboratory study

Substrate type:

other: Clean quartz sand

Limit test:

no

Remarks:

The test ended until at least 65 percent of the control seeds had germinated and developed roots that were at least 20mm long.

Test temperature:

19.0 to 20.0 °C for lettuce
24.5 to 26.0 °C for others

pH:

6.722 to 8.212

Moisture:

5.23 and 8.35% at begining of the test

Details on test conditions:

TEST SYSTEM
- Testing facility: Artiticial climate box
- Amount of soil: Clean quartz sand (about 220 g)
- Method of seeding: The test plant seeds exposed in the test substrate surface. stamped and put into artificial climate chamber.
- No. of seeds per container: 10
- No. of plants (retained after thinning): 10
- No. of replicates per treatment group: 3
- No. of replicates per control: 3

GROWTH CONDITIONS
- Condition: incubated in the dark, culture temperature of lettuce was 19.0 to 20.0°C,other plant cultivation temperatures was 24.5 to 26.0°C.
- Application: Clean quartz sand (about 220 g) were added to homemade inert plastic boxes, then 60-65mL test solution was poured to mixed to prepare the test matrix.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The number of seeds that germinated were counted and root lengths were measured for each test species.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5-2
- Range finding study
- Test concentrations: 0.1%, 1%, 10% and 100% of the saturated solution
- Results used to determine the conditions for the definitive study: 10 kinds of plant seeds germination and root elongation inhibition rate occured between 1% to 100% water-saturated solution.

Nominal and measured concentrations:

Nominal: 1.56%, 3.12%, 6.25%, 12.5%, 25%, 50% and 100% of the saturated solution
Measured: 138, 332, 684, 1440, 2975, 5700 and 12700 mg/L

Reference substance (positive control):

no

Species:

Glycine max (G. soja)

Dose descriptor:

EC50

Effect conc.:

10 440 other: mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

germination

Remarks on result:

other: The test was ended until the germination rate of the control group reached 65% and the average root length was 20 mm

Species:

Lycopersicon esculentum

Dose descriptor:

EC50

Effect conc.:

852 other: mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

germination

Remarks on result:

other: The test was ended until the germination rate of the control group reached 65% and the average root length was 20 mm

Species:

Daucus carota

Dose descriptor:

EC50

Effect conc.:

2 282 other: mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

germination

Remarks on result:

other: The test was ended until the germination rate of the control group reached 65% and the average root length was 20 mm

Species:

Cucumis sativus

Dose descriptor:

EC50

Effect conc.:

9 057 other: mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

germination

Remarks on result:

other: The test was ended until the germination rate of the control group reached 65% and the average root length was 20 mm

Species:

Brassica oleracea var. capitata

Dose descriptor:

EC50

Effect conc.:

724 other: mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

germination

Remarks on result:

other: The test was ended until the germination rate of the control group reached 65% and the average root length was 20 mm

Species:

other: Capsicum frutescans

Dose descriptor:

EC50

Effect conc.:

2 332 other: mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

germination

Remarks on result:

other: The test was ended until the germination rate of the control group reached 65% and the average root length was 20 mm

Species:

Oryza sativa

Dose descriptor:

EC50

Effect conc.:

10 791 other: mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

germination

Remarks on result:

other: The test was ended until the germination rate of the control group reached 65% and the average root length was 20 mm

Key result

Species:

Lactuca sativa

Dose descriptor:

EC50

Effect conc.:

69 other: mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

germination

Remarks on result:

other: The test was ended until the germination rate of the control group reached 65% and the average root length was 20 mm

Species:

Triticum aestivum

Dose descriptor:

EC50

Effect conc.:

3 290 other: mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

germination

Remarks on result:

other: The test was ended until the germination rate of the control group reached 65% and the average root length was 20 mm

Species:

Zea mays

Dose descriptor:

EC50

Effect conc.:

1 954 other: mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

germination

Remarks on result:

other: The test was ended until the germination rate of the control group reached 65% and the average root length was 20 mm

Details on results:

Seed germination and root elongation inhibition effects of 10 test plant were as follows:
Soybean(Glycine max)
EC10 inhibition of germination was 6358 mg/L, EC50 was 10440 mg/L;
EC10 inhibition of root elongation was 412 mg/L (95% confidence limits was 342-1119 mg/L), EC50 inhibition of root elongation was 3398 mg/L (95% confidence limits was 211- 6227 mg/L).
Tomato (Lycperwason esculeatum)
EC10 inhibition of germination was 129 mg/L, EC50 inhibition of germination was 852 mg/L (95% confidence limits was 437-1340 mg/L);
EC10 inhibition of root elongation was 44.0 mg/L, EC50 inhibition of root elongation was 2306 mg/L (95% confidence limits was 724-11188 mg/L).
Carrot(Daucus carota)
EC10 inhibition of germination was 724 mg/L (95% confidence limits was 194-1418 mg/L), EC50 was 2282mg/L (95% confidence limits was 1106-4450 mg/L);
EC10 inhibition of germination was 513 mg/L, EC50 inhibition of root elongation was 3823 mg/L (95% confidence limits was 2601-4.434 mg/L)
Cucumber (Cucumis sativus)
EC10 inhibition of germination was 6674 mg/L (95% confidence limits was 258-9006 mg/L), EC50 was 9057 mg/L(95% confidence limits was 1780-10921 mg/L);
EC10 inhibition of germination was 117 mg/L, EC50 inhibition of root elongation was 2529 mg/L (95% confidence limits was 2017-2980 mg/L).
Cabbage(Brassica oleracea)
EC50 inhibition of germination was 172 mg/L, EC50 was 724 mg/L (95% confidence limits was 331-1374 mg/L);
EC10 inhibition of root elongation was 37.5 mg/L, EC50 was 322 mg/L (95% confidence limits was 102-1718 mg/L)
Peper(Capsicum frotescans)
EC10 inhibition of germination was 219 mg/L, EC50 inhibition of germination was 2332 mg/L (95% confidence limits was 1089-4009 mg/L)
EC10 inhibition of root elongation was 117 mg/L, EC50 inhibition of root elongation was 2137 mg/L
Rice (Oryza sativa)
EC10 inhibition of germination was 974 mg/L, EC50 inhibition of germination was 10791 mg/L;
EC10 inhibition of root elongation was 513 mg/L, EC50 inhibition of root elongation was 5343 mg/L.
Lettuce(Lactuca saliva)
EC10 inhibition of germination was 1.35 mg/L, EC50 inhibition of germination was 69.0 mg/L;
EC10 inhibition of root elongation was 214 mg/L, EC50 inhibition of root elongation was 483 mg/L
Wheat (Triticum aestivurn)
EC10 inhibition of germination was 2003 mg/L (95% confidence limits was 1308-2466 mg/L), EC50 was 3290 mg/L (95% confidence limits was 2749-3814 mg/L).
EC10 inhibition of root elongation was 60 mg/L, EC50 was 1089 mg/L (95% confidence limits was 387-1833 mg/L).
Corn (Zea mays)
EC10 inhibition of germination was 637 mg/L (95% confidence limits was 247-1126 mg/L).EC50 was 1954 mg/L (95% confidence limits was 1102-3368 mg/L);
EC10 inhibition of root elongation was 1299 mg/L (95% confidence limits was 295-1828 mg/L), EC50 was 2223 mg/L (95% confidence limits was 1838-2758 mg/L).

Reported statistics and error estimates:

Analysis (Shapiro-Wik's Test, Dunnett's Test et al.) by software Toxcalc v5.0.26.

Validity criteria fulfilled:

yes

Conclusions:

Seed germination and root elongation inhibition effects of 10 test plant were tested. And the most sensitive species was Lettuce(Lactuca saliva). EC10 inhibition of germination was 1.35 mg/L, EC50 inhibition of germination was 69.0 mg/L; EC10 inhibition of root elongation was 214 mg/L, EC50 inhibition of root elongation was 483 mg/L.

Executive summary:

Seed germination and root elongation toxicity of test substance to Glycine max, Lycperison esculeafum, Daucus carota, Cucumis sativus, 8rassica oleracea, Capsicum frutescans, Oryza saliva, Laetuca sativa, Triticum aestivum and Zea mays were determined according to SEPA 299.

The test concentrations were 1.56%, 3.12%, 6.25%, 12.5%, 25%, 50% and 100% of the saturated solution and the measured concentrations were 138, 332, 684.1440, 2975, 5700 and 12700 mg/L of each concentration is provided with three parallel, each parallel using 10 seeds. When the germination rate of the control group reached 65% and the average root length was 20 mm, the trial reached the end point.

The most sensitive species was Lettuce(Lactuca saliva), and EC10 inhibition of germination was 1.35 mg/L, EC50 inhibition of germination was 69.0 mg/L; EC10 inhibition of root elongation was 214 mg/L, EC50 inhibition of root elongation was 483 mg/L.

Description of key information

Short-term study:

The most sensitive species was Lettuce(Lactuca saliva), and EC10 inhibition of germination was 1.35 mg/L, EC50 inhibition of germination was 69.0 mg/L; EC10 inhibition of root elongation was 214 mg/L, EC50 inhibition of root elongation was 483 mg/L.

Additional information

Seed germination and root elongation toxicity of test substance to Glycine max, Lycperison esculeafum, Daucus carota, Cucumis sativus, 8rassica oleracea, Capsicum frutescans, Oryza saliva, Laetuca sativa, Triticum aestivum and Zea mays were determined according to SEPA Guideline 299.

The test concentrations were 1.56%, 3.12%, 6.25%, 12.5%, 25%, 50% and 100% of the saturated solution and the measured concentrations were 138, 332, 684.1440, 2975, 5700 and 12700 mg/L of each concentration is provided with three parallel, each parallel using 10 seeds. When the germination rate of the control group reached 65% and the average root length was 20 mm, the trial reached the end point.

The most sensitive species was Lettuce(Lactuca saliva), and EC10 inhibition of germination was 1.35 mg/L, EC50 inhibition of germination was 69.0 mg/L; EC10 inhibition of root elongation was 214 mg/L, EC50 inhibition of root elongation was 483 mg/L.