Cyclopentane, methoxy-

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

fish, juvenile growth test

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 2013-01-28 to 2013-06-04

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 215 (Fish, Juvenile Growth Test)

Version / remarks:

2000

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Batch No.: 2820246
Purity: ≥ 99.9%

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 12.5, 25, 50, 100, 200 mg/L
- Sampling method: For analysis of the test concentration, 30 mL of test solution was taken from each test vessel at the first week before and after each renewal, twice a week before and after renewal in the following weeks and at the end of the test.
- Sample storage conditions before analysis: analyzed directly without storage

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 64.0 g, 64.0 g, 64.1 g and 64.0 g test substance were dissolved in test water to prepare the stock solution of 8 g/L. Then based on the measured concentrations, the test solutions were prepared by dilution of the stock solution with test water.
- Controls: blank control of test water

Test organisms (species):

other: Gobiocypris rarus

Details on test organisms:

TEST ORGANISM
- Common name: Chinese Rare Minnow
- Source: Shanghai Academy of Public Measurement
- Weight at study initiation (mean and range, SD): 0.091 g ± 0.011 g (Mean±SD)
- Method of breeding: fish were fed with Brine Shrimp Aquarium Flake Food twice a day and food was withhold from the fish for 24 hours before weighing at the start of the test.

FEEDING DURING TEST
- Feeding:fish were fed twice a day and two feeds were separated by 6 hours. The quantity of food given to fish was with a rate of about 4% of body weight per day. Food was withheld from the fiish for 24 hours prior to weighing.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

28 d

Hardness:

170 mg CaCO3/L in the control
186 mg CaCO3/L in the highest concentration

Test temperature:

22.8-23.7 °C

pH:

7.02-7.85

Dissolved oxygen:

60.2% - 93.8% ASV

Nominal and measured concentrations:

Nominal: 12.5, 25, 50, 100, 200 mg/L
Measured: 13.5, 25.4, 50.7, 95.6 and 196 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10L glass bottle
- Type: closed
- Aeration: no
- Renewal rate of test solution: 24 h
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.18-0.28 g fish/litre darning the test

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The tap water dechlorinated by activated carbon and sterilized by UV lamp was used. The water was aerated continuously over 24h.
- Total hardness: 155 mg CaCO3 per liter;
- Oxygen concentration: >90% of ASV.


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: Light: Dark= 12 hours:12 hours

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The fish were inspected and recorded once a day for mortality and other observed effects. Dead fish were removed when observed and the feeding rate was adjusted.
Total hardness was measured in the controls and one vessel at the highest concentration once during the test.
Measurements of pH, dissolved oxygen and temperature were carried out before and after each renewal.

Reference substance (positive control):

no

Key result

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

50.7 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

28 d

Dose descriptor:

LOEC

Effect conc.:

95.6 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

- Test concentration: In the test, the measured values were within ± 20% of the mean concentrations in each replicate.
- Body weight: In the test, the body weights of the test fish increased in all groups.
- - Growth rate: The specific growth rate of the 95.0 mg/L and 196 mg/L treatment groups were significantly lower than that of the control (p<0.05), the specific growth rate of other treatment groups were not significantly different from that of the control.
- Mortality and abnormality: During the test, there were no mortality and abnormality on fish in treatment groups.

Reported statistics and error estimates:

To determine the lowest observed effect concentration (LOEC) and the no observed effect concentration (NOEC), the variances among the means of the specific growth rate in all groups was analyzed by one-way ANOVA and the mean of treatment groups was compared with the control group using Bonferroni t test. The data treatment was carried out with software TOXCALC (v5.0.26). The results were expressed as geometic mean average of measured concentration of solutions.

Validity criteria fulfilled:

yes

Conclusions:

The no observed effect concentration (NOEC) was 50.7 mg/L and the lowest observed effect concentration (LOEC) was 95.6 mg/L (expressed as the geometric mean measured concentration of test solutions).

Executive summary:

The effect on growth of juvenile fish of the test substance to Chinese Rare Minnow (Gobiocypris rarus} was determined by a closed semi-static test according to OECD 215.

In the test, five treatment groups and a control group were performed. The nominal concentrations of the treatment groups were 12.5 mg/L, 25 mg/L, 50 mg/L,100 mg/L and 200 mg/L(which the mean measured concentrations of the treatment groups were 13.5 mg/L, 25.4 mg/L, 50.7 mg/L, 95.6 mg/L, 196 mg/L). All treatment groups and control group had no replicates with 20 fish and the test duration was 28 days.

In the test, the measured values were within ± 20% of the mean concentrations in each replicate.

During the test, there were no mortality and abnormality on fish in treatment groups.

The no observed effect concentration (NOEC) was 50.7 mg/L and the lowest observed effect concentration (LOEC) was 95.6 mg/L (expressed as the geometric mean measured concentration of test solutions).

Description of key information

The no observed effect concentration (NOEC) was 50.7 mg/L and the lowest observed effect concentration (LOEC) was 95.6 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

50.7 mg/L

Additional information

The effect on growth of juvenile fish of the test substance to Chinese Rare Minnow (Gobiocypris rarus) was determined by a closed semi-static test according to OECD 215.

In the test, five treatment groups and a control group were performed. The nominal concentrations of the treatment groups were 12.5 mg/L, 25 mg/L, 50 mg/L,100 mg/L and 200 mg/L(which the mean measured concentrations of the treatment groups were 13.5 mg/L, 25.4 mg/L, 50.7 mg/L, 95.6 mg/L, 196 mg/L). All treatment groups and control group had no replicates with 20 fish and the test duration was 28 days.

In the test, the measured values were within ± 20% of the mean concentrations in each replicate.

During the test, there were no mortality and abnormality on fish in treatment groups.

The no observed effect concentration (NOEC) was 50.7 mg/L and the lowest observed effect concentration (LOEC) was 95.6 mg/L (expressed as the geometric mean measured concentration of test solutions).