Alcohols, C16-18

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Alcohols, C16-18 is a member and is from Long Chain Alcohols (C6-22 primary aliphatic alcohols) category.
The Long Chain Alcohols (C6-22 primary aliphatic alcohols) category is considered suitable as a source of data for Alcohols, C16-18.
Considered valid for read-across for purposes of classification.
No further vertebrate testing can be justified.

Long Chain Alcohols (C6-22 primary aliphatic alcohols) category covers a family of 30 primary aliphatic alcohols within a carbon chain length range of C6-C22. Commercial products generally include several aliphatic alcohol components, with a range of carbon chain lengths present. The family consists of alcohols with varying compositions and structures. Composition depends on the route to manufacture and the related feedstocks. Most of the alcohols have linear carbon chains but certain manufacturing processes create branched structures. Data are also available for eleven other similar substances, which support the category. Non-sponsored alcohols may not be HPV or may not be produced by members of the consortium, but have structures similar to sponsored linear alcohols.

Key points are that the members share:
• The same structural features
• Similar metabolic pathways
• Common mode of ecotoxicological action
• Common levels and mode of human health related effects.

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

the test substance was not determined in solution

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

yes

Remarks:

the test substance was not determined in solution

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)

- Method: 40 mg of test substance was dissolved in 10 ml of vehicle with the aid of ultrasonic disruption. An aliquot (2 ml) of this stock solution was dispersed in 20 L of dechlorinated tap water to give the test concentration of 0.4 mg/L.

- Chemical name of vehicle: tetrahydrofuran

- Concentration of vehicle in test medium: 10 ml final volume in stock solution (equivalent to 0.1 ml in test medium).

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM

- Common name: rainbow trout

- Source: Donnington Fish Farm, Upper Swell, Gloucestershire, UK


- Length at study initiation (mean and SD): 4.5 cm (s.d = 0.2) at the end of the definitive study

- Weight at study initiation (mean and SD): 1.20 g (s.d = 0.26) at the end of the definitive study

- Feeding during test: feeding was discontinued 24 h prior to test initiation and they were not fed during the test



ACCLIMATION

- Acclimation period: from 26th Feb 1996 to 4th March 1996

- Acclimation conditions: test conditions

- Type and amount of food: commercial trout pellets

- Health during acclimation (any mortality observed): 2%

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

none reported

Hardness:

136 CaCO3 mg/L

Test temperature:

13-14 ºC

pH:

range: 7.5-7.9

Dissolved oxygen:

>= 9.7 mg/L

Salinity:

Not Applicable

Nominal and measured concentrations:

Nominal concentration: 0.4 mg/L

Details on test conditions:

TEST SYSTEM

- Test vessel:

- Type (delete if not applicable): covered to reduce evaporation

- Material, size, headspace, fill volume: 20 L glass vessels


- Renewal rate of test solution (frequency): daily

- No. of organisms per vessel: 10

- No. of vessels per concentration (replicates): 2

- No. of vessels per control (replicates): 1

- No. of vessels per vehicle control (replicates): 1

- Biomass loading rate: 0.6 mg body weight/L (static volume)



TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: dechlorinated tap water (by passage through an activated carbon filter)

- Metals: 70 ug/L or less

- Chlorine: 0.22 mg/L

- Alkalinity: 80 mg/L as CaCO3

- Ca/mg ratio: ca. 6:1

- Conductivity: 382 MICSM

- Culture medium different from test medium: same water

- Intervals of water quality measurement: 0, 24, 48, 72 and 96 h


OTHER TEST CONDITIONS

- Photoperiod: 16 h light, 8 h darkness


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : at 3, 6, 24, 48, 72 and 96 h fish were monitored for mortality and adverse effects (mortality and behaviour).


TEST CONCENTRATIONS

- Spacing factor for test concentrations: 1 test concentrations used

- Justification for using less concentrations than requested by guideline: limit test

- Range finding study

- Test concentrations: 0.04 and 0.4 mg/L

- Results used to determine the conditions for the definitive study: LC50 >0.4 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 0.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: non-toxic at the limit of solubility

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: non-toxic at the limit of solubility

Details on results:

- Mortality of control: 0%

- Effect concentrations exceeding solubility of substance in test medium: The solubility of octadecanol is 0.0011 mg/L at 25oC

Validity criteria fulfilled:

yes

Conclusions:

A 96 h LC50 value of >0.4 mg/L was determined for the effect of the test substance on mortality of the fish O. mykiss. The water solubility of octadecanol is 0.0011 mg/L at 25oC , therefore the LC50 value is >0.0011 mg/L, The water solubility of octadecanol is 0.0011 mg/L at 25oC, therefore the LC50 value is >0.0011 mg/L (>LoS), i.e. the test substance was not toxic at the LoS.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Alcohols, C16-18 is a member and is from Long Chain Alcohols (C6-22 primary aliphatic alcohols) category.
The Long Chain Alcohols (C6-22 primary aliphatic alcohols) category is considered suitable as a source of data for Alcohols, C16-18.
Considered valid for read-across for purposes of classification.
No further vertebrate testing can be justified.

Long Chain Alcohols (C6-22 primary aliphatic alcohols) category covers a family of 30 primary aliphatic alcohols within a carbon chain length range of C6-C22. Commercial products generally include several aliphatic alcohol components, with a range of carbon chain lengths present. The family consists of alcohols with varying compositions and structures. Composition depends on the route to manufacture and the related feedstocks. Most of the alcohols have linear carbon chains but certain manufacturing processes create branched structures. Data are also available for eleven other similar substances, which support the category. Non-sponsored alcohols may not be HPV or may not be produced by members of the consortium, but have structures similar to sponsored linear alcohols.

Key points are that the members share:
• The same structural features
• Similar metabolic pathways
• Common mode of ecotoxicological action
• Common levels and mode of human health related effects.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

yes

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM

- Common name: rainbow trout

- Strain: Oncorhynchus mykiss

- Source: Donnington fish Farm, Upper Swell, Gloucestershire, UK

- Length at study initiation (length definition, mean, range and SD): 4.5 cm (SD = 0.2)

- Weight at study initiation (mean and range, SD): 1.2 g (SD = 0.26)

- Feeding during test: no the animals were starved from 24 h prior to the study initiation


ACCLIMATION

- Acclimation period: 7 days

- Acclimation conditions (same as test or not): yes

- Type and amount of food: commercial trout pellets

- Health during acclimation (any mortality observed): 2% mortality

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

none reported

Hardness:

none reported

Test temperature:

none reported

pH:

range: 7.4-7.9

Dissolved oxygen:

none reported

Salinity:

Not Applicable

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: non-toxic at the limit of solubility

Details on results:

Five acute toxicity tests were conducted with hexadecanol using four different preparation methods. None of the tests resulted in significant mortality due to the action of hexadecanol. Although the deaths in the control (typicaly 10-30%) complicate the interpretation, the data demonstrate that the 96 hour LC50 was greater than the highest concentration tested (10 mg/L). This is about 1000 times the water solubility of hexadecanol.

Validity criteria fulfilled:

yes

Conclusions:

A 96 h LC50 >10 mg/L has been determined for the effects of hexadecanol on the mortality of the freshwater fish Brachydanio rerio (new name: Danio rerio). However this result is above the LoS (limit of solubility), therefore the substance is non-toxic at the limit of LoS and LC50 is >LoS.

Executive summary:

Five acute toxicity tests were conducted with hexadecanol using four different preparation methods. None of the tests resulted in significant mortality due to the action of hexadecanol. Although the deaths in the control (typicaly 10-30%) complicate the interpretation, the data demonstrate that the 96 hour LC50 was greater than the highest concentration tested (10 mg/L). This is about 1000 times the water solubility of hexadecanol.

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Alcohols, C16-18 is a member and is from Long Chain Alcohols (C6-22 primary aliphatic alcohols) category.
The Long Chain Alcohols (C6-22 primary aliphatic alcohols) category is considered suitable as a source of data for Alcohols, C16-18.
Considered valid for read-across for purposes of classification.
No further vertebrate testing can be justified.

Long Chain Alcohols (C6-22 primary aliphatic alcohols) category covers a family of 30 primary aliphatic alcohols within a carbon chain length range of C6-C22. Commercial products generally include several aliphatic alcohol components, with a range of carbon chain lengths present. The family consists of alcohols with varying compositions and structures. Composition depends on the route to manufacture and the related feedstocks. Most of the alcohols have linear carbon chains but certain manufacturing processes create branched structures. Data are also available for eleven other similar substances, which support the category. Non-sponsored alcohols may not be HPV or may not be produced by members of the consortium, but have structures similar to sponsored linear alcohols.

Key points are that the members share:
• The same structural features
• Similar metabolic pathways
• Common mode of ecotoxicological action
• Common levels and mode of human health related effects.

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Principles of method if other than guideline:

The protocol followed in the experiment also took into account the OECD guideline 23 for testing difficult substances and mixtures (2000)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)

- Method: An amount of test material (40 mg) was dissolved in tetrahydrofuran with the aid of ultrasonic disruption and the volume adjusted to 10 ml. An aliquot (2.0 ml) of this solution was dispersed in 20L of dechlorinated tap water to give the concentration of 0.4 mg/L.

- Chemical name of vehicle (organic solvent, emulsifier or dispersant): tetrahydrofuran

- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 2 ml in 20 L, the solvent control group was exposed to 100 ul/L of solvent control.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM

- Common name: rainbow trout

- Strain: Oncorhynchus mykiss

- Source: Donnington fish Farm, Upper Swell, Gloucestershire, UK

- Length at study initiation (length definition, mean, range and SD): 4.5 cm (SD = 0.2)

- Weight at study initiation (mean and range, SD): 1.2 g (SD = 0.26)

- Feeding during test: no the animals were starved from 24 h prior to the study initiation


ACCLIMATION

- Acclimation period: 7 days

- Acclimation conditions (same as test or not): yes

- Type and amount of food: commercial trout pellets

- Health during acclimation (any mortality observed): 2% mortality

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

none reported

Hardness:

136 CaCO3 mg/L

Test temperature:

14 degC

pH:

range: 7.4-7.9

Dissolved oxygen:

greater or equal to 9.3 mg O2/L

Salinity:

Not Applicable

Nominal and measured concentrations:

Nominal concentrations: 0.4 mg/L

Details on test conditions:

TEST SYSTEM

- Test vessel:

- Type (delete if not applicable): covered

- Material, size, headspace, fill volume: 20 L glass tanks

- Aeration: yes, via narrow bore glass tube

- Renewal rate of test solution (frequency/flow rate): 24 hours

- No. of organisms per vessel: 10

- No. of vessels per concentration (replicates): 2

- No. of vessels per control (replicates): 1

- No. of vessels per vehicle control (replicates): 1


TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: dechlorinated tap water

- Alkalinity: 80 mg/L

- Conductivity: 405 uS/cm

- Culture medium different from test medium: no

- Intervals of water quality measurement: daily


OTHER TEST CONDITIONS

- Photoperiod: 8 h dark, 16 h light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and sub-lethal effects were recorded at 3, 6, 24, 48 and 96 hours.


TEST CONCENTRATIONS

- Justification for using less concentrations than requested by guideline: the test substance was tested up to the limit of solubility.

- Range finding study

- Test concentrations: 0.1 and 1.0 mg/L

- Results used to determine the conditions for the definitive study: 96h EC50 >1.0 mg/L

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 0.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: non-toxic at the limit of solubility

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: non-toxic at the limit of solubility

Details on results:

- Mortality of control: 0%

- Effect concentrations exceeding solubility of substance in test medium: the test was conducted above the limit of solubility of the test substance, with the employment of the solvent tetrahydrofuran. The presence of undissolved material was not reported.

Sublethal observations / clinical signs:

No mortalities (0%) were observed in the control, solvent control and 1.0 mg/L groups after 96 h. Therefore the LC50 could not be determined. The water solubility of the test substance is 0.013 mg/l at 25 degC [Yalkowsky et al, 1992]. Therefore the test substance was non-toxic at the limit of solubility.

Validity criteria fulfilled:

yes

Conclusions:

A 96 h LC50 >0.4 mg/L has been determined for the effects of hexadecanol on the mortality of the freshwater fish O. mykiss. However this result is above the LoS (limit of solubility), therefore the substance is non-toxic at the limit of LoS and LC50 is >LoS.This is about 1000 times the water solubility of the test substance.

Reference 4

Endpoint:

short-term toxicity to fish

Type of information:

(Q)SAR

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Justification for type of information:

QSAR prediction:US EPA accepted QSAR method for chemicals properties assessment.

Qualifier:

according to guideline

Guideline:

other: QSAR

Principles of method if other than guideline:

EPI Suite v 4.1/ECOSARv1.00

GLP compliance:

no

Remarks:

not applicable to QSAR models

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: Fishes species

Details on test organisms:

no further information

Test type:

other: QSAR model

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

not applicable. QSAR model

Test temperature:

not applicable. QSAR model

pH:

not applicable. QSAR model

Dissolved oxygen:

not applicable. QSAR model

Salinity:

not applicable. QSAR model

Details on test conditions:

Alcohols, C16-18
CAS Number: 67762-27-0
SMILES : CCCCCCCCCCCCCCCCO
CHEM : Alcohols, C16-18
MOL FOR: C16 H34 O1
MOL WT : 242.45
Log Kow: 6.73 (KowWin estimate)
Wat Sol: 0.0412 mg/L (experimental database)

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.012 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: (Q)SAR prediction

Remarks on result:

other: non-toxic at the limit of solubility, as determined by (Q)SAR

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: (Q)SAR prediction

Remarks on result:

other: Predicted, at water solubility loading rates of 1000 mg/L

Details on results:

A 96 h LC50 value of 0.012 mg/L has been calculated for the effects of the test substance on the mortality of the marine fish taxonomic group. This result signifies that the test substance is not expected to be toxic at the limit of solubility.
A reliable 96 h LC50 value of 120 mg/L has been calculated for the effects of the test substance towards fish taxonomic group. Therefore the test substance has been predicted to be non toxic at the limit of solubility, so the LC50 is 0.012 mg/L. Predicted to be non-toxic at the limit of solubility. Predicted to be non-toxic at the limit of solubility.
The water solubility of the Alcohols, C16-18 is 0.0412 mg/l at 25 degC (experimental database). This is about 10000 times the water solubility of the test substance.

Results with reference substance (positive control):

A 96 h LC50 value of 0.012 mg/L has been calculated for the effects of the test substance on the mortality of the marine fish taxonomic group. This result signifies that the test substance is not expected to be toxic at the limit of solubility.

Sublethal observations / clinical signs:

Predicted to be non-toxic at the limit of solubility

Validity criteria fulfilled:

yes

Remarks:

US EPA accepted QSAR method for chemicals properties assessment.

Conclusions:

A 96 h LC50 value of 0.012 mg/L has been calculated for the effects of the test substance on the mortality of the marine fish taxonomic group. This result signifies that the test substance is not expected to be toxic at the limit of solubility.This result is above the LoS (limit of solubility), therefore the substance is non-toxic at the limit of LoS and LC50 is >LoS.
A reliable 96 h LC50 value of 120 mg/L has been calculated for the effects of the test substance towards fish taxonomic group. Therefore the test substance has been predicted to be non toxic at the limit of solubility, so the LC50 is 0.012 mg/L. Predicted to be non-toxic at the limit of solubility. Predicted to be non-toxic at the limit of solubility.

Executive summary:

The ECOSAR QSAR model predicts that the 96hr LC50 for maine fish would to be 0.012 mg/l .This result signifies that the test substance is not expected to be toxic at the limit of solubility.This result is above the LoS (limit of solubility), therefore the substance is non-toxic at the limit of LoS and LC50 is >LoS. The water solubility of the Alcohols, C16-18 is 0.0412 mg/l at 25 degC (experimental database). This is about 10000 times the water solubility of the test substance.

Reference 5

Endpoint:

short-term toxicity to fish

Type of information:

(Q)SAR

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Justification for type of information:

QSAR prediction: US EPA accepted QSAR method for chemicals properties assessment.

Qualifier:

according to guideline

Guideline:

other: QSAR

Principles of method if other than guideline:

EPI Suite v 4.1/ECOSARv1.00

GLP compliance:

no

Remarks:

not applicable to QSAR models

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: Fishes species

Details on test organisms:

no further information

Test type:

other: QSAR model

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

not applicable. QSAR model

Test temperature:

not applicable. QSAR model

pH:

not applicable. QSAR model

Dissolved oxygen:

not applicable. QSAR model

Salinity:

not applicable. QSAR model

Details on test conditions:

Alcohols, C16-18
CAS Number: 67762-27-0
SMILES : CCCCCCCCCCCCCCCCO
CHEM : Alcohols, C16-18
MOL FOR: C16 H34 O1
MOL WT : 242.45
Log Kow: 6.73 (KowWin estimate)
Wat Sol: 0.0412 mg/L (experimental database)

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.013 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: (Q)SAR prediction

Remarks on result:

other: non-toxic at the limit of solubility, as determined by (Q)SAR

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

130 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: (Q)SAR prediction

Remarks on result:

other: Predicted, at an water solubility loading rates of 1000 mg/L

Details on results:

A 96 h LC50 value of 0.013 mg/L has been calculated for the effects of the test substance on the mortality of the fish taxonomic group. This result signifies that the test substance is not expected to be toxic at the limit of solubility.
A reliable 96 h LC50 value of 130 mg/L has been calculated for the effects of the test substance towards fish taxonomic group.Therefore the test substance has been predicted to be non toxic at the limit of solubility, so the LC50 is 0.013 mg/L. Predicted to be non-toxic at the limit of solubility.
The water solubility of the Alcohols, C16-18 is 0.0412 mg/l at 25 degC (experimental database).This is about 10000 times the water solubility of the test substance.

Results with reference substance (positive control):

A 96 h LC50 value of 0.013 mg/L has been calculated for the effects of the test substance on the mortality of the fish taxonomic group. This result signifies that the test substance is not expected to be toxic at the limit of solubility.

Validity criteria fulfilled:

yes

Remarks:

US EPA accepted QSAR method for chemicals properties assessment.

Conclusions:

A 96 h LC50 value of 0.013 mg/L has been calculated for the effects of the test substance on the mortality of the fish taxonomic group. This result signifies that the test substance is not expected to be toxic at the limit of solubility. This result is above the LoS (limit of solubility), therefore the substance is non-toxic at the limit of LoS and LC50 is >LoS.
A reliable 96 h LC50 value of 130 mg/L has been calculated for the effects of the test substance towards fish taxonomic group. Therefore the test substance has been predicted to be non toxic at the limit of solubility, so the LC50 is 0.013 mg/L. Predicted to be non-toxic at the limit of solubility.

Executive summary:

The ECOSAR QSAR model predicts that the 96hr LC50 for freshwater fish would to be 0.013 mg/l .This result signifies that the test substance is not expected to be toxic at the limit of solubility.This result is above the LoS (limit of solubility), therefore the substance is non-toxic at the limit of LoS and LC50 is >LoS. The water solubility of the Alcohols, C16-18 is 0.0412 mg/l at 25 degC (experimental database). This is about 10000 times the water solubility of the test substance.

Description of key information

A reliable 96 h LC50 value of 130 mg/L has been calculated for the effects of the test substance towards freshwater fish taxonomic group. Therefore the test substance has been predicted to be non toxic at the limit of solubility, so the LC50 is 0.013 mg/L. Predicted to be non-toxic at the limit of solubility.
This result signifies that the test substance is not expected to be toxic at the limit of solubility.This result is above the LoS (limit of solubility), therefore the substance is non-toxic at the limit of LoS and LC50 is >LoS. The water solubility of the Alcohols, C16-18 is 0.0412 mg/l at 25 degC (experimental database). This is about 10000 times the water solubility of the test substance.
A reliable 96 h LC50 value of 120 mg/L has been calculated for the effects of the test substance towards marne fish taxonomic group. Therefore the test substance has been predicted to be non toxic at the limit of solubility, so the LC50 is 0.012 mg/L. Predicted to be non-toxic at the limit of solubility. 
This result signifies that the test substance is not expected to be toxic at the limit of solubility.This result is above the LoS (limit of solubility), therefore the substance is non-toxic at the limit of LoS and LC50 is >LoS. The water solubility of the Alcohols, C16-18 is 0.0412 mg/l at 25 degC (experimental database). This is about 10000 times the water solubility of the test substance.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

130 mg/L

Marine water fish

Marine water fish

Dose descriptor:

LC50

Effect concentration:

120 mg/L

Additional information

A reliable 96 h LC50 value of 130 mg/L has been calculated for the effects of the test substance towards freshwater fish taxonomic group. Therefore the test substance has been predicted to be non toxic at the limit of solubility, so the LC50 is 0.013 mg/L. Predicted to be non-toxic at the limit of solubility.

This result signifies that the test substance is not expected to be toxic at the limit of solubility.This result is above the LoS (limit of solubility), therefore the substance is non-toxic at the limit of LoS and LC50 is >LoS. The water solubility of the Alcohols, C16-18 is 0.0412 mg/l at 25 degC (experimental database). This is about 10000 times the water solubility of the test substance.

A reliable 96 h LC50 value of 120 mg/L has been calculated for the effects of the test substance towards marne fish taxonomic group. Therefore the test substance has been predicted to be non toxic at the limit of solubility, so the LC50 is 0.012 mg/L. Predicted to be non-toxic at the limit of solubility.

This result signifies that the test substance is not expected to be toxic at the limit of solubility.This result is above the LoS (limit of solubility), therefore the substance is non-toxic at the limit of LoS and LC50 is >LoS. The water solubility of the Alcohols, C16-18 is 0.0412 mg/l at 25 degC (experimental database). This is about 10000 times the water solubility of the test substance.