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EC number: 807-754-7
CAS number: 89929-57-7
7.2.2/1: Exposure Chamber Concentration
actual concentration of the test item was measured off-line by high
performance gas chromatography (GC). The test atmospheres were sampled
after theoretical chamber equilibration and then at approximately
half-hourly intervals during each exposure period.
concentration of the test item was shown to be stable and the mean
values obtained were:
Mean Achieved (mg/L)
chamber flow rate was maintained at 60 L/min providing 120 air changes
theoretical chamber equilibration time (T99) was 3 minutes (Silver,
7.2.2/2: Particle Size Distribution
Mean Achieved Atmosphere Concentration (mg/L)
Mean Mass Median Aerodynamic Diameter (μm)
(% <4 μm)
Geometric Standard Deviation
7.2.2/3: Mortality Data
an acute inhalation toxicity study performed according to OECD Guideline
403 and in compliance with GLP, three
groups of five or ten RccHan™ : WIST strain rats (five males and/or five
females) were exposed to an aerosol atmosphere of the test item for 4
concentration: 21.9, 11.9 and 17.2 mg/L (corresponding to mean achieved
atmosphere concentrations: 5.05, 3.25 and 4.62 mg/L, respectively)
were observed for mortality, clinical signs and body weight for 14 days
and at the end of the study the animals were sacrificed for macroscopic
5.05 mg/L, 1/5 male and 3/5 females died;
3.25 mg/L, 0/5 female died (males not exposed);
4.62 mg/L, 2/5 females died (males not exposed).
abnormalities noted during the study included decreased respiratory
rate, increased respiratory rate, hunched posture, pilo-erection, and
wet fur. There were frequent instances of labored respiration,
occasional instances of noisy respiration, ataxia, coma and red/brown
staining around the eyes and snout and isolated occurrences of
exophthalmos, dehydration, pallor of the extremities, lethargy, ptosis
and prostration. Surviving Group 1 animals appeared normal on Day 7
post-exposure. All Group 2 animals recovered to appear normal on Day 3
post-exposure and surviving animals from Group 3 recovered to appear
normal on Day 6 post-exposure.
1 (5.05 mg/L) – All surviving animals exhibited body weight losses on
Day 1 post-exposure. Body weight gains were then noted in all surviving
animals during the remainder of the recovery period.
2 (3.25 mg/L) – All animals exhibited body weight losses on the first
day post-exposure. With the exception of one animal, which showed no
body weight gain during the final week of the recovery period, body
weight gains were noted in all animals during the remainder of the
3 (4.62 mg/L) – All surviving animals exhibited body weight losses on
Day 1 post-exposure and from Days 1 to 3 post-exposure. Body weight
gains were then noted in all animals during the remainder of the
the exception of one male animal from Group 1 which exhibited dark
patches on the lungs, no macroscopic abnormalities were detected at
necropsy amongst animals that survived until the end of the recovery
periods. The following macroscopic abnormalities were detected at
necropsy amongst animals that were humanely killed or were found dead
during the course of the study: Lungs – dark patches; Small
intestine – contained dark substance.
to the observations noted during the study and at necropsy it is
considered that deaths noted during the study may have been mainly
attributable to systemic toxicity.
inhalation LC50 and 95% confidence limits of test item were calculated
animals: 5.23 (4.45 – 6.01) mg/L; Males only: 5.18 (3.98 – 6.38) mg/L;
Females only: 4.84 (4.29 – 5.38) mg/L
the test conditions, the test item is classified as Category 4 according
to Regulation (EC) No. 1272/2008 (CLP) and to GHS.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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