Amines, C12-14-alkyl, C6-10-alkyl phosphates

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

The test item was tested for toxicity towards daphnia as aqueous extracts ("WAF") from up to 100 mg test item/L (final concentration).

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS (clone 5) of Iabaratory bred (derived from Dr. M. Bergtold, BASF, D-67117 Limburgerhof; the strain originally derived from the Institut National de Recherche Chimique Appliquee, France in 1978 and cultivated since then at BASF using medium M4 acc. Elendt) were used for the test. ln order to verify the sensitivity of the test system towards toxic items the effect of potassium dichromate towards different animals of this bred was tested prior to beginning of the test. The value of the EC50 (24h) in this period of time was 1.13 mg/L potassium dichromate. This value is in accordance with the recommendations given in OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test). The cultivation of the daphnia is performed in a way that the animals are transferred in new test medium (see below) at an interval of 1 to 3 days followed by feeding with Desmodesmus subspicatus- and/or Chlorella-cells. Daphnia used for the test are not older than 24h. Therefore, in every case, daphnia are transferred into fresh medium one day before starting of the daphnia test in order to get daphnia for the test younger than 24h. During the test the daphnia were not fed.

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

48 h

Test temperature:

ca 21 °C

pH:

ca 8.5

Dissolved oxygen:

5.9 - 8.6 mg O2/L

Reference substance (positive control):

yes

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 0.315 mg/L

Nominal / measured:

nominal

Conc. based on:

other: "WAF", water accommodated fraction

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 0.2 mg/L

Nominal / measured:

nominal

Conc. based on:

other: "WAF", water accommodated fraction

Basis for effect:

mobility

10-1.2 Main Test Loading of the test item (mg/L) (Eluates/"WAF")	Ratio immobilised Daphnia [^0/0]      24 h                 48h		pH-Value to                48h		02-Content [mg 02/L] to            48h		Temperature to               48h
Control (0) 0.1 0.2 0.4 0.8 1 .0	o [0/20]^1)o [0/20]^1)o [0/20]^1) 40 [8/20]1) 90 [1 8/20]^1) 100 [20/20]^1)	5 [1/20]^1)o [0/20]^1) 5 [1/20]^1) 95 [1 9/20]^1) 100 [20/20]^1) 100 [20/201^1)	8.51 8.48 8.49 8.47 8.57 8.59	8.54 8.25 8.41 8.35 8.33 8.53	8.6 6.2 5.9 6.1 5.7 6.1	8.3 7.7 7.1 7.3 6.0 6.5	21 .0 21 .0 21 .0 21.o 21.0 21.o	19.9 20.0 20.0 20.0 20.1 20.2

1) Numbers in brackets give the absolute numbers of immobilised animals at each concentration.

Validity criteria fulfilled:

yes

Conclusions:

"DEOPHOS 228" was tested for acute toxicity towards Daphnia according to OECD-Test Guideline 202. ln order to investigate the influence of the test item towards the daphnia, the swimming behaviour of the animals was recorded. Under the conditions used for the test, immobilisation of the daphnia was observed at concentrations representing the water soluble part (hereafter abbreviated "WAF", water accommodated fraction) of the test item of ~0.1 mg/L. The following EL-values (effective loading rate) are assumed according to the results of this test:
t24h t48h
NOEC No precise value is given by the system 0.20 mg/L
LOEC No value given by the system 0.40 mg/L
EL1o 0.284 mg/L (0.222 - 0.364) 0.261 mg/L (no Iimits given)
EL2o 0.334 mg/L (0.272 - 0.411) 0.278 mg/L (no Iimits given)
EL5o 0.456 mg/L (0.389- 0.535) 0.315 mg/L (no Iimits given)
Values in brackets indicate the 95% confidence Iimits. The test results are based on the nominal concentrations being tested as the test item was measured to be stable at in the aqueous phase at ~80% during incubation. The criteria of validity of results given by the OECD-Test Guideline 202 (version dated 13-Apr-2004) were kept within this study:
• ln the control without any test item no dead animals were observed.
• ln the test solutions the oxygen Goncentration did not drop below a value of 60% of the saturation
value ("' 5 mg 02/L).

Executive summary:

The test substance was tested for acute toxicity towards Daphnia according to OECD-Test Guideline 202. ln order to investigate the influence of the test item towards the daphnia, the swimming behaviour of the animals was recorded. Under the conditions used for the test, immobilisation of the daphnia was observed at concentrations representing the water soluble part (hereafter abbreviated "WAF", water accommodated fraction) of the test item of ~0.1 mg/L. The following EL-values (effective loading rate) are assumed according to the results of this test:

t24h                                                        t48h

NOEC               No precise value is given by the system                    0.20 mg/L

LOEC               No value given by the system                                   0.40 mg/L

EL1o                 0.284 mg/L (0.222 - 0.364)                                   0.261 mg/L (no Iimits given)

EL2o                 0.334 mg/L (0.272 - 0.411)                                   0.278 mg/L (no Iimits given)

EL5o                 0.456 mg/L (0.389- 0.535)                                   0.315 mg/L (no Iimits given)

Values in brackets indicate the 95% confidence Iimits. The test results are based on the nominal concentrations being tested as the test item was measured to be stable in the aqueous phase at ~80% during incubation.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.315 mg/L

Additional information

The test substance was tested for acute toxicity towards Daphnia according to OECD-Test Guideline 202. ln order to investigate the influence of the test item towards the daphnia, the swimming behaviour of the animals was recorded. Under the conditions used for the test, immobilisation of the daphnia was observed at concentrations representing the water soluble part (hereafter abbreviated "WAF", water accommodated fraction) of the test item of ~0.1 mg/L. The following EL-values (effective loading rate) are assumed according to the results of this test:

t24h                                                        t48h

NOEC               No precise value is given by the system                    0.20 mg/L

LOEC               No value given by the system                                   0.40 mg/L

EL1o                 0.284 mg/L (0.222 - 0.364)                                   0.261 mg/L (no Iimits given)

EL2o                 0.334 mg/L (0.272 - 0.411)                                   0.278 mg/L (no Iimits given)

EL5o                 0.456 mg/L (0.389- 0.535)                                   0.315 mg/L (no Iimits given)

Values in brackets indicate the 95% confidence Iimits. The test results are based on the nominal concentrations being tested as the test item was measured to be stable in the aqueous phase at ~80% during incubation.