Amines, C12-14-alkyl, C6-10-alkyl phosphates

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

no analytical monitoring carried out due to absence of valid method

Justification for type of information:

Refer to the section 13 of IUCLID dataset for details on the read across justification. The short term toxicity study to fish with the read across substance is considered sufficient to fulfil the information requirements as further explained in the provided endpoint summary.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

not specified

Remarks:

The study was conducted in 1984 in a lab according to OECD Guideline 203, however no mention of GLP conditions were reported in the study report.

Analytical monitoring:

no

Vehicle:

yes

Remarks:

ethylene glycol monomethyl ether

Details on test solutions:

500 mg of test substance were mixed with 30 mg ARKOPAL N 150 and made up to 50 mL with ethylene glycol monomethylether.

Test organisms (species):

other: Salmo gairdneri

Details on test organisms:

Length : 50.2 mm
Weight : 0.96 g
Loading : 0.6 g/L
Acclimatisation: 14 d
Supplier : P. Hohler / CH-4314 Zeiningen

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

145 mg CaCO3/L

Test temperature:

15 degree Celsius

pH:

8.1

Dissolved oxygen:

91-104 % saturation

Nominal and measured concentrations:

Nominal: 0, 0.58, 1, 1.8, 3.2 and 5.8 mg/L

Details on test conditions:

Vessels : Glass aquaria of 20 L filled with 15 L (36/22/25 cm)
Number of fishes: 10 fishes per concentration and control, 5 fishes per aquarium
Aeration: gentle aeration during the test
Lighting: Fluorescent light, 16 h daily
Measurements: pH, O2,temperature daily

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

5.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not specified

Conclusions:

Under the study conditions, the nominal 96 h LC50 of the test substance in rainbow trout was determined to be 5.5 mg/L.

Executive summary:

A study was conducted to determine the short term toxicity of the read-across substance amines, C11 -14 -branched alkyl, monohexyl and dihexyl phosphates to fish according to OECD Guideline 203. A static exposure study was conducted in rainbow trout (Salmo gairdneri) at nominal concentrations of 0, 0.58, 1, 1.8, 3.2 and 5.8 mg/L of the test substance. The test substance stock solution was prepared in ethylene glycol monomethyl ether. The analytical determination of the test concentrations was not possible due to unavailability of a valid method at the test facility. Ten test fish per concentration were used at a loading rate of 0.6 g/L in 15 L of water medium in glass aquarium of 20 L capacity. pH, O2 and temperature were monitored throughout the 96 h exposure duration. Under the study conditions, the nominal 96 h LC50 of the test substance in rainbow trout was determined to be 5.5 mg/L (Rufli, 1984).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

5.5 mg/L

Additional information

A study was conducted to determine the short term toxicity of the read-across substance amines, C11 -14 -branched alkyl, monohexyl and dihexyl phosphates to fish according to OECD Guideline 203. A static exposure study was conducted in rainbow trout (Salmo gairdneri) at nominal concentrations of 0, 0.58, 1, 1.8, 3.2 and 5.8 mg/L of the test substance. The test substance stock solution was prepared in ethylene glycol monomethyl ether. The analytical determination of the test concentrations was not possible due to unavailability of a valid method at the test facility. Ten test fish per concentration were used at a loading rate of 0.6 g/L in 15 L of water medium in glass aquarium of 20 L capacity. pH, O2 and temperature were monitored throughout the 96 h exposure duration. Under the study conditions, the nominal 96 h LC50 of the test substance in rainbow trout was determined to be 5.5 mg/L (Rufli, 1984).