Registration Dossier
Registration Dossier
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EC number: 246-836-1 | CAS number: 25321-14-6
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Reliability as cited in OECD SIDS Dinitrotoluene (isomers mixture) CAS No.: 25321-14-6, 2004. Meets generally accepted scientific standards, limited documentation, acceptable for assessment
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
- Principles of method if other than guideline:
- 50 mg of test substance were instilled in the left eye of rabbits.
- GLP compliance:
- no
Test material
- Reference substance name:
- Dinitrotoluene
- EC Number:
- 246-836-1
- EC Name:
- Dinitrotoluene
- Cas Number:
- 25321-14-6
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- Reaction mass of 1-methyl-2,4-dinitrobenzene and 2-methyl-1,3-dinitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): dinitrotoluene
- Analytical purity: ca. 80% 2,4-DNT and 20% 2,6-DNT
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
Test system
- Vehicle:
- water
- Controls:
- not required
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 mg
- Concentration (if solution): aqueous paste - Duration of treatment / exposure:
- substance remained in the eye for a maximum of 7 days.
- Observation period (in vivo):
- 7 days; examination time points: 0, 24, 48, 72 hours and 7 days
- Number of animals or in vitro replicates:
- 2
- Details on study design:
- SCORING SYSTEM:
Examination of cornea, iris, erythema and oedema. No data on scoring system.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- other: all observation timepoints
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicalbe
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- other: all observation timepoints
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: not applicable
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- other: all observation time points
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- other: not applicable
Any other information on results incl. tables
At 24 hours slight erythema in one animal. Score of 0 for all other observation timepoints.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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