Registration Dossier
Registration Dossier
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EC number: 246-836-1 | CAS number: 25321-14-6
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Reliability as cited in OECD SIDS Dinitrotoluene (isomers mixture) CAS No.: 25321-14-6, 2004. Comparable to guideline study with acceptable restrictions (limited documentation)
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
- Reference Type:
- secondary source
- Title:
- Dinitrotoluene (isomers mixture), CAS No.: 25321-14-6
- Author:
- OECD SIDS
- Year:
- 2 004
- Bibliographic source:
- OECD SIDS
Materials and methods
- Principles of method if other than guideline:
- Male rats received a single oral dose by gavage. Post observation period of 14 days.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Dinitrotoluene
- EC Number:
- 246-836-1
- EC Name:
- Dinitrotoluene
- Cas Number:
- 25321-14-6
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- Reaction mass of 1-methyl-2,4-dinitrobenzene and 2-methyl-1,3-dinitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): dinitrotoluene 80/20
- Analytical purity: no data
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Züchter Winkelmann
- Weight at study initiation: 160 - 180 g
- Housing: 5 per cage
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- polyethylene glycol
- Doses:
- 100, 400, 800, 1500 and 2000 mg/kg
- No. of animals per sex per dose:
- 10
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Other examinations performed: clinical signs, body weight
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 660 mg/kg bw
Any other information on results incl. tables
Mortality: 0/10, 4/10, 5, 10, 7/10 and 10/10 animals died at 100, 400, 800, 1500 and 2000 mg/kg, respectively. Time of death was 3 -7 days at 400 mg/kg, 3 h - 5 days at 800 mg/kg, 2 - 6 days at 1500 mg/kg and 2 - 3 days at 2000 mg/kg.
Clinical signs: 10/10 animals in the 400, 800, 1500 and 2000 mg/kg dose groups showed clinical signs. Clinicals signs noted were diuresis, diarrhea, weight loss, shaggy fur, bloody nose, disturbance of equilibrium and loss of hair.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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