Dinitrotoluene

Toxicity to soil microorganisms

Administrative data

Endpoint:

toxicity to soil microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: test procedure in accordance with national standard methods

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: BBA Nr. VI, 1-1 (1990): Dehydrogenase activity of soil microflora

Principles of method if other than guideline:

Method developed by "Biologische Bundesanstalt, Braunschweig (Germany)"

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dinitrotoluene

EC Number:

204-450-0

EC Name:

2,4-dinitrotoluene

Cas Number:

121-14-2

Molecular formula:

C7H6N2O4

IUPAC Name:

1-methyl-2,4-dinitrobenzene

Details on test material:

- Name of test material (as cited in study report): 2,4-Dinitrotoluene (2,4-DNT)
- Analytical purity: 99%

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

not specified

Test organisms

Test organisms (inoculum):

soil

Study design

Total exposure duration:

28 d

Test conditions

Test temperature:

22°C

Moisture:

ca. 60% of maximum field capacity

Details on test conditions:

- natural soil microorganisms were tested; 
- two soils were used ( sandy soil: pH 6.8; loamy soil: pH 7.3-7.4); 
- incubation in the dark

Nominal and measured concentrations:

5 and 50 mg/kg soil dw

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

28 d

Dose descriptor:

other: EC13

Effect conc.:

5 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: dehydrogenase activity

Remarks on result:

other: loamy soil

Effect concentrations 2

Duration:

28 d

Dose descriptor:

other: EC43

Effect conc.:

50 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: dehydrogenase activity

Remarks on result:

other: loamy soil

Any other information on results incl. tables

Reduction of dehydrogenase activity of 
a. sandy soil microorganisms: 26-46% during the whole
exposure period and at both concentrations tested;

b. loamy soil microorganisms: at the beginning of the test,
no effect was observed with a concentration of 5 mg/kg,
whereas a concentration of 50 mg/kg caused an inhibition of
32%;
inhibition was highest at the 14th day of exposure at both
concentrations tested (60-70%); at the end of the test,
inhibition had declined to 13 and 43% at 2,4-DNT
concentrations of 5 and 50 mg/kg, respectively.