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EC number: 219-702-5 | CAS number: 2500-88-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July - Oct 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Scientifically acceptable; study conform to OECD Guideline 476 with minor deviation; GLP study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Dioctadecyl disulphide
- EC Number:
- 219-702-5
- EC Name:
- Dioctadecyl disulphide
- Cas Number:
- 2500-88-1
- Molecular formula:
- C36H74S2
- IUPAC Name:
- dioctadecyl disulphide
- Reference substance name:
- Di-n-octadecyl disulphide
- IUPAC Name:
- Di-n-octadecyl disulphide
- Reference substance name:
- Hostanox SE 10
- IUPAC Name:
- Hostanox SE 10
Constituent 1
Constituent 2
Constituent 3
Method
- Target gene:
- Thymidine Kinase Lokus of L5178Y TK+/- mouse lymphoma cells
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 Homogenate
- Test concentrations with justification for top dose:
- 600, 800, and 1000 µg/ml two cultures (tested in triplicate each)
75, 150, 300, 600, 800 and 1000 µg/ml two cultures (tested in triplicate each) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: ethanol
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- Remarks:
- without metabolic activation
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- N-dimethylnitrosamine
- Remarks:
- with metabolic activation
- Details on test system and experimental conditions:
- The test material appeared soluble in the assay medium up to about 300 µg/ml. The test was performed up to the dose level of 1000 µg/ml
- Evaluation criteria:
- Assay Acceptance Criteria:
1. The average absolute cloning effiency of the negative controls should be between 70 and 130%.
All assays below 50% cloning efficiency are unacceptable.
2. The solvent and untreated controls normally have the same growth rates and cloning efficiencies within experimental error. An assys will be unacceptable if the average percent relative growth of the solvent controls is less than about 70% of the untreated control value.
3. The minimum acceptable value for the suspension growth of the average negtive control (average of the solvent and untreated control value) for two days is 8.0.
4. The background mutant frequency is calculated separately for concurrent activation and nonactivation. For both conditions, the normal range of background frequencies for assays performed with differen cell stocks is 5 x 10(exp)-6 to 60 x 10(exp)-6. Assays with backgrounds outside this range are not necessarily invalid but will not be used as primary evidence for the evaluation of a test material.
5. An assay will be acceptable in the absence of a positive control only if the test material clearly shows mutagenic activity. If the test material appaers to have no or only weak mutagenic activity, an acceptablee assay must have a positive control mutant frequency above the lower limits of the normal range.
6. For test materials with little or no mutagenic activity, an assay must include applied concentrations that reduce the suspension growth to 10 to 15% of the average solvent control or reach the maximum applied concnetrations given in the corresponding guideline.
7. An experimental treatment that results in fewer than 2.5 x 10(exp)6 cells by the end of the two-day growth period will not be cloned for mutant analysis.
8. An experimental mutant frequency will be considered accpetable for evaluation only if the relative cloning efficiency is 10% or greater and the total number of viable clones exceeds about 20. - Statistics:
- None
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- ADDITIONAL INFORMATION ON CYTOTOXICITY: No significant toxic effect observed up to the highest dose level which was well above the solubility limit of the test susbtance in the test medium
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
The test substance Hostanox SE 10 is not mutagenic in Mouse Lymphoma Assay. - Executive summary:
The test substance was tested for mutagenicity using mammalian cells L5178Y TK+/- with and without metabolic activation in the dose range of 75-1000 µg/ml. The highest dose level was well above the solubility limit of the test substance in the test medium and obvious cell toxicity was not observed. No mutagenic activity was found.
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