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EC number: 201-070-7
CAS number: 77-93-0
The toxicity to aquatic algae was assessed in a test with Pseudokirchnerella subcapitata (reported as Selenastrum capricornutum) according to OECD 201 (2010):EC50 (72h) > 100 mg/L (based on growth rate and yield)NOEC (72h) ≥ 100 mg/L (based on growth rate and yield)LOEC (72h) > 100 mg/L (based on growth rate and yield)
The acute toxicity to aquatic algae and
cyanobacteria of triethyl citrate was tested according to OECD Guideline
201 and EU Method C.3 in an Algal Growth Inhibition Limit Test with the
freshwater green algae Pseudokirchneriella subcapitata (formerly
known as Selenastrum capricornutum) as test organism (2010). The
study was conducted under certificated GLP compliance. The only
concentration tested was nominal 100 mg test item/L. Additionally, a
control was tested in parallel (test water without addition of the test
item). Thus a limit test was performed in accordance with the OECD 201
to demonstrate that the test item has no inhibitory effect on the growth
of the test organism up to this concentration. The study encompassed 2
treatment groups (1 dose rate of the test item, control) with six
replicates per test concentration and six replicates for the control. At
test start 50 mL of the test concentrations were inoculated with 5000
algal cells per mL test medium. These cells were taken from an
exponentially growing pre-culture, which was set up 4 days prior to the
test start under the same conditions as in the test. All calculations
were carried out using a statistical software programme. Analysis of the
yield and growth rate of the algal population within 72 h exposure
period gave the following results: The NOErC (72h) and the NOEyC
(72h) were determined to be at least nominal 100 mg test item/L. The
NOEC values might even be higher than this concentration, but
concentrations in excess of nominal 100 mg test item/L or the limit of
solubility have not been tested according to the recommendations of the
test guidelines. The LOErC (72h), the LOEyC (72h),
the ErC50 (72h) and the EyC50 were clearly higher
than nominal 100 mg test item/L (mean measured: clearly higher than 72
mg test item/L). No toxic effects against algae were observed at the
limit of water solubility under these exposure conditions.
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