2-chloroaniline

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Documentation is insufficient for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

; different volumes applied, reporting deficiencies concerning housing, body weights, signs of toxicity

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-chloroaniline

EC Number:

202-426-4

EC Name:

2-chloroaniline

Cas Number:

95-51-2

Molecular formula:

C6H6ClN

IUPAC Name:

2-chloroaniline

Details on test material:

IUCLID4 Test substance: no data
- Name of test material (as cited in study report): o-Chloranilin
- Physical state: liquid
- Analytical purity: no data

Test animals

Species:

rat

Strain:

other: Wistar-II

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Winkelmann
- Weight at study initiation: 160-180 g
- Housing: 5 per cage

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: 1.6 mL/ kg

Doses:

0.5, 0.7, 0.9, 1.0, 1.6 ml/kg body weight

No. of animals per sex per dose:

10 male rats per dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: day of dosing every hour and once a day thereafter until day 7 post application and then again on day 14
- Necropsy of survivors performed: yes/no
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other:

Statistics:

The LD50 was calculated by the method of Fink and Hund (1965)

Results and discussion

Preliminary study:

no data

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

1 016 mg/kg bw

95% CL:

883 - 1 186

Remarks on result:

other: density 1.21 mg/ cm³; no constant volumes applied

Mortality:

0.5 mL (605 mg)/ kg /bw: 0/10
0.7 mL (847 mg)/ kg /bw: 4/10; 3h-2d post-application
0.9 mL (1089 mg)/ kg /bw: 6/10; 2d post-application
1.0 mL (1210 mg)/ kg /bw: 6/10; 3h-2d post-application
1.6 mL (1936 mg)/ kg /bw: 6/10; 1h-2d post-application

Clinical signs:

other: 0.5 mL (605 mg)/ kg /bw: sedation, convulsion, chromodakryorrhoe 0.7 mL (847 mg)/ kg /bw: sedation, convulsion 0.9 mL (1089 mg)/ kg /bw: sedation, convulsion, chromodakryorrhoe 1.0 mL (1210 mg)/ kg /bw: sedation, convulsion, face down position 1.6 mL (193

Gross pathology:

no data