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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
eye irritation: in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Although the study has been conducted according to GLP guidelines, no final report was drafted. As the data summary lacks detail on: identity/composition of test material, materials and methods which makes it difficult to interpret the available data and to reproduce the study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
no details on the test method protocol followed were included in the report. Although the information is limited, it appears that the test procedure is in line with the HET-CAM test method protocol recommended by ICCVAM in 2006.
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
N-[3-(dimethylamino)propyl]acrylamide
EC Number:
223-342-4
EC Name:
N-[3-(dimethylamino)propyl]acrylamide
Cas Number:
3845-76-9
Molecular formula:
C8 H16 N2 O
IUPAC Name:
N‐[3‐(dimethylamino)propyl]prop‐2‐enamide
Details on test material:
In the study report no information is provided about the exact composition of the material tested. However, the DIMAPA test sample was taken at the end of the production process at "Ringleitung P10" which is the same sampling point as used for substance analytical identification.

Test animals / tissue source

Species:
other: in vitro: Hen's Egg Test Chorioallantoic Membrane (HET-CAM)

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not specified
Amount / concentration applied:
200 µl
Observation period (in vivo):
0.5 min
Number of animals or in vitro replicates:
Chorioallantoic Membranes of 4 different eggs

Results and discussion

In vitro

Results
Irritation parameter:
other: HET-CAM score range
Run / experiment:
Mean
Value:
12
Remarks on result:
other: Time point: 0.5 min; Max score: 21
Other effects / acceptance of results:
Irritant/corrosive response data: 12/21

Any other information on results incl. tables

         Lysis        hemorrhage        coagulation
 egg #  0.5'  2'  5'  0.5'  2'  5'  0.5'  2'  5'
 1  5  - -  7 -  -  -   - - 
 2  5 -  -  7  -   - -   - - 
 3  5 -  -  7  -  -  -   - - 
 4  5 -  -  7 -   - -   - - 
 total  20 -  -  28  -  -  -   - - 

Applicant's summary and conclusion

Interpretation of results:
highly irritating
Remarks:
Migrated information Criteria used for interpretation of results: not specified
Conclusions:
Severe eye irritation
Executive summary:

A HET-CAM study was performed on 4 chicken embryos (10d, 50-60 g eggs). Based on Lysis and Hemorrhage an avarage total score of 12/21 was observed which corresponds to "severely irritating to eyes".