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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study with analytical test item concentration verification

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report Date:
1993

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2,6-DCPTFMA
- Physical state: browm liquid
- Analytical purity: 89.7 %
- Lot/batch No.: 51 ADM92
- Storage condition of test material: dark at room temperature (approximately 20°C).

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Source: salmonids hatchery - Pisciculture TALLET, Le petit Large, 84800 Saumane, France.
- Length at study initiation (length definition, mean, range and SD): 6.0 (5.5 - 6.4) cm
- Weight at study initiation (mean and range, SD): 2.4 (1.8 - 3.2) g
- Feeding during test: Yes


ACCLIMATION
- Conditions equivalent to housing
- Health during acclimation (any mortality observed): _Three fish mortality of 291 fish during the last 7 days prior to test initiation

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
Total hardness: about 220 mg/L CaCO3
Test temperature:
12.7 - 13.9 °C
pH:
7.4 - 8.1
Dissolved oxygen:
7.6 - 9.2 mg/L
Nominal and measured concentrations:
nominal: 0.26, 0.32, 0.50, 0.62 and 0.78 mg/L
percentag of nominal after 96 h: - 35 %
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: all-glass aquaria, 20 L
- Renewal rate of test solution (frequency/flow rate): Fixed flow rates of stock solutions were 3.41, 2.69, 2.15, 1.71, 1.37 and 1.11 ml/min. for
the nominal concentrations of 0.78, 0.62, 0.50, 0.40, 0.32 and 0.26 mg/l respectively.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): two
- No. of vessels per control (replicates): two
- Biomass loading rate: 0.19 g/L


OTHER TEST CONDITIONS
- Photoperiod: 12 hours light : 12 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and symptoms


TEST CONCENTRATIONS
- Spacing factor for test concentrations: approximately 1.25

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.11 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion