Tetra(sodium/potassium)-7-[(E)-{2-acetamido-4-[(E)-(4-{[4-chlor-6-({2-[(4-fluor-6-{[4-(vinylsulfonyl)phenyl]amino}-1,3,5-triazin-2-yl)amino]propyl}amino)-1,3,5-triazin-2-yl]amino}-5-sulfonato-1-naphthyl)diazenyl]-5-methoxyphenyl}diazenyl]-1,3,6-naphthalintrisulfonate

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experiment start date - 28 June 2006; Experiment end date - 29 June 2006; Study completion date - 16 October 2006.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Specific details on test material used for the study:

Identity: FAT 40827/A
Batch: T2 5572 BOP 01/06
Purity: determined in this study
Appearance: black sticky powder
Expiration date: 28.02.2011
Storage: at room temperature

Radiolabelling:

no

Test temperature:

Temperature: 25 °C

Details on study design: HPLC method:

Reagents and Apparatus:
Methanol: Baker, Art. No. 8402
Water: RCC, from Milli-Q water supply
Balance: Mettler, XP 205 DR
pH meter: Metrohm 692 with Metrohm electrode 6.0258.000

HPLC-Conditions:
Apparatus: Varian autosampler 9095
Varian Pump 9012
Varian UV/VIS detector ProStar 310
Jones column oven 7990
Column: LiChrospher 100 CN, particle size 5 μm, 250 mm x 4 mm
Pre-column: CN
Mobile phase: Water / methanol (45:55; v/v); pH 6.7
Detection: Test item: 254 nm
Reference items: 210 nm
Sodium nitrate: 210 nm
Temperature: 25 °C
Injection volume: 10 μl
Flow: 1.0 ml/min
Run time: 20 minutes

Type:

Koc

Value:

< 18

Temp.:

25 °C

  Retention times in minutes of the reference items and the test item:

	1^strun	2^ndrun	3^rdrun	4^thrun	5^thrun	6^thrun
Sodium nitrate (= dead time)	1.173	1.172
Reference Items:
Acetanilide	3.004	3.000	3.002	3.002	3.002	3.001
Isoproturon	3.511	3.506	3.507	3.509	3.510	3.507
Linuron	4.365	4.350	4.358	4.363	4.364	4.360
Pyrazophos	5.586	5.570	5.574	5.582	5.586	5.571
Diclofop-methyl	6.511	6.493	6.497	6.506	6.513	6.495
2,4-DDT	9.967	9.939	9.946	9.954	9.975	9.942
Test Item peak:	0.982	0.982	0.983

Validity criteria fulfilled:

yes

Conclusions:

In conclusion, the log of the adsorption coefficient (KOC) of test item was estimated to be log KOC <1.25 which is equal to a KOC value of <18 using the HPLC method. This value indicates that the test item will not be adsorbed by organic carbon in soil. The test item can be considered to be of very high mobility.

Executive summary:

The determination of the adsorption coefficient (K_OC) of test item was performed according to the OECD Guideline for the Testing of Chemicals, No. 121, Estimation of the Adsorption Coefficient (K_OC) on Soil and Sewage Sludge using High Performance Liquid Chromatography (HPLC), January, 2001 and the EC Directive 2001/59, C.19. The test item solution was injected three times and the reference solution was injected six times. The test item was not retained under the chromatographic conditions of the test and was eluted at the dead time. The K_OC of test item was therefore determined to be lower than that one of the lowest reference item used (acetanilide). The chromatography of the test item resulted in one main peak with a well-defined and reproducible retention time. The HPLC method was therefore considered to be suitable for the purpose of the study. Based on the chromatographic data, the test item was considered to be stable during the test procedure. In conclusion, the log of the adsorption coefficient (K_OC) of test item was estimated to be log K_OC <1.25 which is equal to a K_OC value of < 18 using the HPLC method. This value indicates that the test item will not be adsorbed by organic carbon in soil. The test item can be classified to be of very high mobility according to P. J. McCall, D. A. Laskowski, R. L. Swann and H. J. Dishburger "Measurement of Sorption Coefficients of Organic Chemicals and Their Use in Environmental Fate Analysis"; 1981; Assoc. of Off. Anal. Chem., Arlington, VA; p 89–109.

Description of key information

The log of the adsorption coefficient (KOC) of test item was estimated to be log KOC <1.25 which is equal to a KOC value of <18 using the HPLC method.

Key value for chemical safety assessment

Koc at 20 °C:

18

Additional information

The determination of the adsorption coefficient (K_OC) of test item was performed according to the OECD Guideline for the Testing of Chemicals, No. 121, Estimation of the Adsorption Coefficient (K_OC) on Soil and Sewage Sludge using High Performance Liquid Chromatography (HPLC), January, 2001 and the EC Directive 2001/59, C.19. The test item solution was injected three times and the reference solution was injected six times. The test item was not retained under the chromatographic conditions of the test and was eluted at the dead time. The K_OC of test item was therefore determined to be lower than that one of the lowest reference item used (acetanilide). The chromatography of the test item resulted in one main peak with a well-defined and reproducible retention time. The HPLC method was therefore considered to be suitable for the purpose of the study. Based on the chromatographic data, the test item was considered to be stable during the test procedure. In conclusion, the log of the adsorption coefficient (K_OC) of test item was estimated to be log K_OC<1.25 which is equal to a K_OC value of <18 using the HPLC method. This value indicates that the test item will not be adsorbed by organic carbon in soil. The test item can be classified to be of very high mobility according to P. J. McCall, D. A. Laskowski, R. L. Swann and H. J. Dishburger "Measurement of Sorption Coefficients of Organic Chemicals and Their Use in Environmental Fate Analysis"; 1981; Assoc. of Off. Anal. Chem., Arlington, VA; p 89–109.